Exploiting Endogenous Enzymes for Cancer-Cell Selective Metabolic Labeling of RNA in Vivo.
J Am Chem Soc
; 144(16): 7085-7088, 2022 04 27.
Article
in En
| MEDLINE
| ID: mdl-35416650
Tissues and organs are composed of many diverse cell types, making cell-specific gene expression profiling a major challenge. Herein we report that endogenous enzymes, unique to a cell of interest, can be utilized to enable cell-specific metabolic labeling of RNA. We demonstrate that appropriately designed "caged" nucleosides can be rendered active by serving as a substrate for cancer-cell specific enzymes to enable RNA metabolic labeling, only in cancer cells. We envision that the ease and high stringency of our approach will enable expression analysis of tumor cells in complex environments.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
RNA
/
Neoplasms
Language:
En
Journal:
J Am Chem Soc
Year:
2022
Document type:
Article
Affiliation country:
United States
Country of publication:
United States