[Agkistrodon halys venom antitumor component-I inhibits vasculogenic mimicry in triple-negative breast cancer cells in vitro by down-regulating MMP2].
Nan Fang Yi Ke Da Xue Xue Bao
; 42(3): 438-442, 2022 Mar 20.
Article
in Zh
| MEDLINE
| ID: mdl-35426810
ABSTRACT
OBJECTIVE:
To investigate the inhibitory effect of agkistrodon halys venom antitumor component-I (AHVAC-I) on vasculogenic mimicry (VM) formation in triple-negative breast cancer MDA-MB-231 cells and explore its possible mechanism.METHODS:
CCK8 assay was used to determine the optimal concentration of AHVAC-I for cell treatment based on its halfinhibitory concentration (IC50). MDA-MB-231 cells were treated with different concentrations of AHVAC-I or 5-Fu, and the changes in vasomimetic capacity of the cells were examined using Matrigel assay. The expression levels of matrix metalloproteinase-2 (MMP2) and MMP9 in the treated cells were detected using quantitative PCR and Western blotting.RESULTS:
Compared with the control treatment with culture medium, treatment with 5, 10 and 20 µg/mL AHVAC-I significantly reduced vasomimetic ability of MDA-MB-231 cells in a dose-dependent manner (P < 0.01). MMP2 supplementation obviously restored the vasomimetic ability of the cells inhibited by AHVAC-I.CONCLUSION:
AHVAC-I inhibits VM formation in triplenegative breast cancer cells in vitro by down-regulating MMP2 production.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Agkistrodon
/
Triple Negative Breast Neoplasms
/
Antineoplastic Agents
Limits:
Animals
/
Humans
Language:
Zh
Journal:
Nan Fang Yi Ke Da Xue Xue Bao
Year:
2022
Document type:
Article
Affiliation country:
China