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Rhizobium cremeum sp. nov., isolated from sewage and capable of acquisition of heavy metal and aromatic compounds resistance genes.
Yang, Endong; Liu, Jing; Chen, Di; Wang, Siyi; Xu, Li; Ma, Keke; Zhang, Xinxin; Sun, Leni; Wang, Weiyun.
Affiliation
  • Yang E; School of Life Science, Anhui Agricultural University, Hefei 230036, PR China. Electronic address: yedwill@163.com.
  • Liu J; School of Life Science, Anhui Agricultural University, Hefei 230036, PR China.
  • Chen D; School of Life Science, Anhui Agricultural University, Hefei 230036, PR China.
  • Wang S; School of Life Science, Anhui Agricultural University, Hefei 230036, PR China.
  • Xu L; School of Life Science, Anhui Agricultural University, Hefei 230036, PR China.
  • Ma K; School of Life Science, Anhui Agricultural University, Hefei 230036, PR China.
  • Zhang X; School of Life Science, Anhui Agricultural University, Hefei 230036, PR China.
  • Sun L; School of Life Science, Anhui Agricultural University, Hefei 230036, PR China.
  • Wang W; School of Life Science, Anhui Agricultural University, Hefei 230036, PR China. Electronic address: weiywswzy@163.com.
Syst Appl Microbiol ; 45(3): 126322, 2022 May.
Article in En | MEDLINE | ID: mdl-35427953
Two strains of Rhizobia isolated from sewage collected from the Chinese Baijiu distillery were characterized using a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strains W15T and W16 were grouped as a separate clade closely related to Rhizobium daejeonense L61T (98.6%). Multilocus sequence analysis (MLSA) with three housekeeping genes (recA, glnII and rpoA) also showed that strains W15T and W16 belonged to the genus Rhizobium. Average nucleotide identity and digital DNA-DNA hybridization values between genome sequences of strain W15T and the closely related species ranged from 77.0% to 87.8% and from 23.9% to 30.9%. The DNA G + C content of strain W15T was 61.6 mol%. Strain W15T contained Q-10 as the major ubiquinone and the dominant fatty acids were summed feature 8 (C 18:1ω7c and/or C 18:1ω6c; 73.1%) and C18:0 (7.6%). The main polar lipids are phosphatidylcholine, phosphatidylmethylethanolamine, phosphatidylethanolamine and phosphatidylglycerol. On the basis of the evidences presented in this study, strains W15T and W16 represents a novel species of the genus Rhizobium, for which the name Rhizobium cremeum sp. nov. is proposed. The type strain is W15T (= CGMCC 1.18731T = KACC 22344T).
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Rhizobium / Metals, Heavy Language: En Journal: Syst Appl Microbiol Year: 2022 Document type: Article Country of publication: Germany

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Rhizobium / Metals, Heavy Language: En Journal: Syst Appl Microbiol Year: 2022 Document type: Article Country of publication: Germany