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Suppression of MIR31HG affects the functional properties of thyroid cancer cells depending on the miR-761/MAPK1 axis.
Peng, Shuwang; Chen, Luyang; Yuan, Zhengtai; Duan, Shanshan.
Affiliation
  • Peng S; Department of Gastrointestinal and Thyroid and Vascular Surgery, The First Hospital of Hunan University of Chinese Medicine, Ward 22, 13th floor, Zhihe Building, No.95 Shaoshan Middle Road, Yuhua District, Changsha, 410000, Hunan, Province, China. pengshuwang@126.com.
  • Chen L; Department of Ultrasound Imaging, The First Hospital of Hunan University of Chinese Medicine, Changsha, Hunan, China.
  • Yuan Z; Department of Gastrointestinal and Thyroid and Vascular Surgery, The First Hospital of Hunan University of Chinese Medicine, Ward 22, 13th floor, Zhihe Building, No.95 Shaoshan Middle Road, Yuhua District, Changsha, 410000, Hunan, Province, China.
  • Duan S; Department of Gastrointestinal and Thyroid and Vascular Surgery, The First Hospital of Hunan University of Chinese Medicine, Ward 22, 13th floor, Zhihe Building, No.95 Shaoshan Middle Road, Yuhua District, Changsha, 410000, Hunan, Province, China.
BMC Endocr Disord ; 22(1): 107, 2022 Apr 20.
Article in En | MEDLINE | ID: mdl-35443670
BACKGROUND: Thyroid cancer is the most prevalent endocrine malignancy. Long non-coding RNA (lncRNA) MIR31HG is abnormally expressed in thyroid cancer tissues. However, the precise, critical role of MIR31HG in thyroid cancer development remains unclear. METHODS: MIR31HG, microRNA (miR)-761 and mitogen-activated protein kinase 1 (MAPK1) were quantified by quantitative real-time PCR (qRT-PCR) and immunoblotting. Cell viability, proliferation, apoptosis, invasion and migration abilities were evaluated by MTS, 5-Ethynyl-2'-Deoxyuridine (EdU), flow cytometry, transwell and wound-healing assays, respectively. Dual-luciferase reporter assays were used to validate the direct relationship between miR-761 and MIR31HG or MAPK1. RESULTS: MIR31HG was overexpressed in human thyroid cancer, and its overexpression predicted poor prognosis. Suppression of MIR31HG impeded cell proliferation, invasion and migration, as well as promoted cell apoptosis in vitro, and diminished the growth of xenograft tumors in vivo. Mechanistically, MIR31HG targeted and regulated miR-761. Moreover, miR-761 was identified as a molecular mediator of MIR30HG function in regulating thyroid cancer cell behaviors. MAPK1 was established as a direct and functional target of miR-761 and MAPK1 knockdown phenocopied miR-761 overexpression in impacting thyroid cancer cell behaviors. Furthermore, MIR31HG modulated MAPK1 expression by competitively binding to miR-761 via the shared binding sequence. CONCLUSION: Our findings demonstrate that MIR31HG targets miR-761 to regulate the functional behaviors of thyroid cancer cells by upregulating MAPK1, highlighting a strong rationale for developing MIR31HG as a novel therapeutic target against thyroid cancer.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Thyroid Neoplasms / MicroRNAs / RNA, Long Noncoding Type of study: Prognostic_studies Limits: Humans Language: En Journal: BMC Endocr Disord Year: 2022 Document type: Article Affiliation country: China Country of publication: United kingdom

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Thyroid Neoplasms / MicroRNAs / RNA, Long Noncoding Type of study: Prognostic_studies Limits: Humans Language: En Journal: BMC Endocr Disord Year: 2022 Document type: Article Affiliation country: China Country of publication: United kingdom