Generation of precision-cut slice cultures of human placenta.
STAR Protoc
; 3(2): 101291, 2022 06 17.
Article
in En
| MEDLINE
| ID: mdl-35463475
ABSTRACT
We present a protocol to generate an advanced ex vivo model of human placenta. We use a vibrating tissue slicer to obtain precision-cut slices representative of the entire thickness of human placenta. This approach delivers standardized cultures with a preserved microstructure and cellular composition comparable to the native tissue. We applied this system to study SARS-CoV-2 infection at the maternal-fetal interface. Moreover, this system can be used to investigate the basic functions of the human placenta in health and disease. For complete details on the use and execution of this protocol, please refer to Fahmi et al. (2021).
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
SARS-CoV-2
/
COVID-19
Limits:
Female
/
Humans
/
Pregnancy
Language:
En
Journal:
STAR Protoc
Year:
2022
Document type:
Article
Affiliation country:
Switzerland