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Generation of precision-cut slice cultures of human placenta.
Fahmi, Amal; Brügger, Melanie; Zumkehr, Beatrice; Oliveira Esteves, Blandina I; Baud, David; Alves, Marco P.
Affiliation
  • Fahmi A; Institute of Virology and Immunology, Bern, Switzerland.
  • Brügger M; Department of Infectious Diseases and Pathobiology, Vetsuisse Faculty, University of Bern, Bern, Switzerland.
  • Zumkehr B; Graduate School for Cellular and Biomedical Sciences, University of Bern, Bern, Switzerland.
  • Oliveira Esteves BI; Institute of Virology and Immunology, Bern, Switzerland.
  • Baud D; Department of Infectious Diseases and Pathobiology, Vetsuisse Faculty, University of Bern, Bern, Switzerland.
  • Alves MP; Institute of Virology and Immunology, Bern, Switzerland.
STAR Protoc ; 3(2): 101291, 2022 06 17.
Article in En | MEDLINE | ID: mdl-35463475
ABSTRACT
We present a protocol to generate an advanced ex vivo model of human placenta. We use a vibrating tissue slicer to obtain precision-cut slices representative of the entire thickness of human placenta. This approach delivers standardized cultures with a preserved microstructure and cellular composition comparable to the native tissue. We applied this system to study SARS-CoV-2 infection at the maternal-fetal interface. Moreover, this system can be used to investigate the basic functions of the human placenta in health and disease. For complete details on the use and execution of this protocol, please refer to Fahmi et al. (2021).
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: SARS-CoV-2 / COVID-19 Limits: Female / Humans / Pregnancy Language: En Journal: STAR Protoc Year: 2022 Document type: Article Affiliation country: Switzerland
Fulltext
Add to My VHL
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: SARS-CoV-2 / COVID-19 Limits: Female / Humans / Pregnancy Language: En Journal: STAR Protoc Year: 2022 Document type: Article Affiliation country: Switzerland