Movement of myosin fragments in vitro: domains involved in force production.
Cell
; 48(6): 953-63, 1987 Mar 27.
Article
in En
| MEDLINE
| ID: mdl-3548997
We have used the Nitella-based movement assay to localize the site of force production in myosin. Methods were developed to use nonfilamentous myosin or proteolytic fragments of myosin in place of the thick filaments used in the original assay. In the experiments described here, the tail of myosin or its subfragments is anchored via antibodies to the surface of small particles. Nonfilamentous myosin or its subfragments move along Nitella actin cables at speeds similar to those obtained with filamentous myosin. We generated short HMM, a myosin fragment containing the heads and only 400 A of the tail. Although short HMM lacks the "hinge" region proposed by Harrington to be the site of force generation, and is incapable of forming thick filaments, it moves along actin at speeds above 1 micron/sec. Therefore, neither a thick filament nor the carboxy-terminal 1100 A of the tail is required for movement along actin. The results indicate that force production occurs in or near the myosin heads.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Peptide Fragments
/
Myosins
Limits:
Animals
Language:
En
Journal:
Cell
Year:
1987
Document type:
Article
Country of publication:
United States