Purification, crystallization and drug screening of the IRAK pseudokinases.

Lange, Sven M; Kulathu, Yogesh

Lange, Sven M; Kulathu, Yogesh.

Affiliation

Lange SM; MRC Protein Phosphorylation and Ubiquitylation Unit, Sir James Black Centre, Dundee, Scotland, United Kingdom. Electronic address: slange001@dundee.ac.uk.
Kulathu Y; MRC Protein Phosphorylation and Ubiquitylation Unit, Sir James Black Centre, Dundee, Scotland, United Kingdom.

Methods Enzymol ; 667: 101-121, 2022.

Article in En | MEDLINE | ID: mdl-35525539

Subject(s)

Immunity, Innate; Signal Transduction; Crystallization; Drug Evaluation, Preclinical

Key words

Biolayer-interferometry (BLI); Cell signaling; Drug discovery; High-throughput screening; IRAK2; IRAK3; Innate immunity; Interleukin-1 receptor (IL1R); Protein crystallization; Protein purification; Pseudokinases; Thermal shift assay; Toll-like receptor (TLR)

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Signal Transduction / Immunity, Innate Type of study: Diagnostic_studies / Screening_studies Language: En Journal: Methods Enzymol Year: 2022 Document type: Article Country of publication: United States

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