Combining multiple fluorescence imaging techniques in biology: when one microscope is not enough.
Mol Biol Cell
; 33(6): tp1, 2022 05 15.
Article
in En
| MEDLINE
| ID: mdl-35549314
ABSTRACT
While fluorescence microscopy has proven to be an exceedingly useful tool in bioscience, it is difficult to offer simultaneous high resolution, fast speed, large volume, and good biocompatibility in a single imaging technique. Thus, when determining the image data required to quantitatively test a complex biological hypothesis, it often becomes evident that multiple imaging techniques are necessary. Recent years have seen an explosion in development of novel fluorescence microscopy techniques, each of which features a unique suite of capabilities. In this Technical Perspective, we highlight recent studies to illustrate the benefits, and often the necessity, of combining multiple fluorescence microscopy modalities. We provide guidance in choosing optimal technique combinations to effectively address a biological question. Ultimately, we aim to promote a more well-rounded approach in designing fluorescence microscopy experiments, leading to more robust quantitative insight.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Biology
/
Optical Imaging
Language:
En
Journal:
Mol Biol Cell
Journal subject:
BIOLOGIA MOLECULAR
Year:
2022
Document type:
Article