Detergent Alternatives: Membrane Protein Purification Using Synthetic Nanodisc Polymers.
Methods Mol Biol
; 2507: 375-387, 2022.
Article
in En
| MEDLINE
| ID: mdl-35773593
ABSTRACT
The development of styrene maleic acid (SMA) and diisobutylene maleic acid (DIBMA) copolymers provides an alternative to traditional detergent extraction of integral membrane proteins. By inserting into the membrane, these polymers can extract membrane proteins along with lipids in the form of native nanodiscs made by poly(styrene co-maleic anhydride) derivatives. Unlike detergent solubilization, where membrane proteins may lose annular lipids necessary for proper folding and stability, native nanodiscs allow for proteins to reside in the natural lipid environment. In addition, polymer-based nanodiscs can be purified using common chromatography methods similar to protocols established with detergent solubilization purification. Here we describe the solubilization screening and purification of an integral membrane protein using several commercial copolymers.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Detergents
/
Membrane Proteins
Language:
En
Journal:
Methods Mol Biol
Journal subject:
BIOLOGIA MOLECULAR
Year:
2022
Document type:
Article
Affiliation country:
United States