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Depot- and diabetes-specific differences in norepinephrine-mediated adipose tissue angiogenesis, vascular tone, collagen deposition and morphology in obesity.
Shen, Lei; Dashwood, Michael R; Casale, Carlo; Orie, Nelson N; Evans, Ian M; Sufi, Pratik; Gray, Rosaire; Mohamed-Ali, Vidya.
Affiliation
  • Shen L; Rayne Building, University College London, London, UK. Electronic address: rmhalsh@ucl.ac.uk.
  • Dashwood MR; Royal Free Campus, University College London, London, UK.
  • Casale C; Rayne Building, University College London, London, UK.
  • Orie NN; Royal Free Campus, University College London, London, UK; Anti-Doping Lab Qatar, Doha, Qatar.
  • Evans IM; Cancer Stem Cell Team, Institute of Cancer Research, London, UK.
  • Sufi P; Whittington Hospital, London, UK.
  • Gray R; Rayne Building, University College London, London, UK; Whittington Hospital, London, UK.
  • Mohamed-Ali V; Royal Free Campus, University College London, London, UK; Anti-Doping Lab Qatar, Doha, Qatar.
Life Sci ; 305: 120756, 2022 Sep 15.
Article in En | MEDLINE | ID: mdl-35780713
ABSTRACT

AIMS:

Norepinephrine (NE) is a known regulator of adipose tissue (AT) metabolism, angiogenesis, vasoconstriction and fibrosis. This may be through autocrine/paracrine effects on local resistance vessel function and morphology. The aims of this study were to investigate, in human subcutaneous and omental adipose tissue (SAT and OAT) NE synthesis, angiogenesis, NE-mediated arteriolar vasoconstriction, the induction of collagen gene expression and its deposition in non-diabetic versus diabetic obese subjects. MATERIALS AND

METHODS:

SAT and OAT from obese patients were used to investigate tissue NE content, tyrosine hydroxylase (TH) density, angiogenesis including capillary density, angiogenic capacity and angiogenic gene expression, NE-mediated arteriolar vasoconstriction and collagen deposition. KEY

FINDINGS:

In the non-diabetic group, NE concentration, TH immunoreactivity, angiogenesis and maximal vasoconstriction were significantly higher in OAT compared to SAT (p < 0.05). However, arterioles from OAT showed lower NE sensitivity compared to SAT (10-8 M to 10-7.5 M, p < 0.05). A depot-specific difference in collagen deposition was also observed, being greater in OAT than SAT. In the diabetic group, no significant depot-specific differences were seen in NE synthesis, angiogenesis, vasoconstriction or collagen deposition. SAT arterioles showed significantly lower sensitivity to NE (10-8 M to 10-7.5 M, p < 0.05) compared to the non-diabetic group.

SIGNIFICANCE:

SAT depot in non-diabetic obese patients exhibited relatively low NE synthesis, angiogenesis, tissue fibrosis and high vasoreactivity, due to preserved NE sensitivity. The local NE synthesis in OAT and diabetes desensitizes NE-induced vasoconstriction, and may also explain the greater tissue angiogenesis and fibrosis in these depots.
Subject(s)
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Norepinephrine / Diabetes Mellitus / Neovascularization, Pathologic Limits: Humans Language: En Journal: Life Sci Year: 2022 Document type: Article

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Norepinephrine / Diabetes Mellitus / Neovascularization, Pathologic Limits: Humans Language: En Journal: Life Sci Year: 2022 Document type: Article