Feruloyl Esterases Protein Engineering to Enhance Their Performance as Biocatalysts: A Review.
Chembiochem
; 23(22): e202200354, 2022 11 18.
Article
in En
| MEDLINE
| ID: mdl-35781918
ABSTRACT
Feruloyl esterases (FAEs) are versatile enzymes able to release hydroxycinnamic acids or synthesize their ester derivatives, both molecules with interesting biological activities such as antioxidants, antifungals, antivirals, antifibrotic, anti-inflammatory, among others. The importance of these molecules in medicine, food or cosmetic industries provides FAEs with several biotechnological applications as key industrial biocatalysts. However, FAEs have some operational limitations that must be overcome, which can be addressed through different protein engineering approaches to enhance their thermal stability, catalytic efficiencies, and selectivity. This review aims to present a brief historical tour through the mutagenesis strategies employed to improve enzymes performance and analyze the current protein engineering strategies applied to FAEs as interesting biocatalysts. Finally, an outlook of the future of FAEs protein engineering approaches to achieve successful industrial biocatalysts is given.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Carboxylic Ester Hydrolases
/
Protein Engineering
Language:
En
Journal:
Chembiochem
Journal subject:
BIOQUIMICA
Year:
2022
Document type:
Article
Affiliation country:
Mexico
Publication country:
ALEMANHA
/
ALEMANIA
/
DE
/
DEUSTCHLAND
/
GERMANY