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Generation of an alpaca serum that induces immune-mediated crescentic glomerulonephritis in mice.
Dhana, Ermanila; Klaus, Daniela; Böhner, Alexander; Schmidt, Florian; von Vietinghoff, Sibylle; Boor, Peter; Djudjaj, Sonja; Kurts, Christian.
Affiliation
  • Dhana E; Institute of Molecular Medicine and Experimental Immunology, Medical Faculty, Rheinische Friedrich-Wilhelms University, Bonn, Germany.
  • Klaus D; Institute of Molecular Medicine and Experimental Immunology, Medical Faculty, Rheinische Friedrich-Wilhelms University, Bonn, Germany.
  • Böhner A; Institute of Molecular Medicine and Experimental Immunology, Medical Faculty, Rheinische Friedrich-Wilhelms University, Bonn, Germany.
  • Schmidt F; Institute of Innate Immunity, University of Bonn, Germany.
  • von Vietinghoff S; Nephrology Section, Medical Clinic 1, University Hospital Bonn, Rheinische Friedrich-Wilhelms University, Bonn, Germany.
  • Boor P; Institute of Pathology, University hospital RWTH, Aachen, Germany.
  • Djudjaj S; Institute of Pathology, University hospital RWTH, Aachen, Germany.
  • Kurts C; Institute of Molecular Medicine and Experimental Immunology, Medical Faculty, Rheinische Friedrich-Wilhelms University, Bonn, Germany. Electronic address: ckurts@uni-bonn.de.
J Immunol Methods ; 507: 113310, 2022 08.
Article in En | MEDLINE | ID: mdl-35787393
ABSTRACT
Crescentic glomerulonephritis (cGN) is the most aggressive form of glomerulonephritis in humans. A widely studied mouse model is induced by sheep or rabbit antisera raised against murine renal cortical antigens. We here, report that Alpaca readily produce ample amounts of antisera that induces pathology in mice, resembling human disease regarding crescent formation, proteinuria, infiltrating immune cells and a significant Th1, but not Th17 immune response. Alpaca antiserum did not cause end-stage kidney failure, neither in a passive nor in an accelerated experimental setting, which may be advantageous for long term studies of crescentic glomerulonephritis.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Camelids, New World / Glomerulonephritis Type of study: Etiology_studies Limits: Animals / Humans Language: En Journal: J Immunol Methods Year: 2022 Document type: Article Affiliation country: Germany
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Camelids, New World / Glomerulonephritis Type of study: Etiology_studies Limits: Animals / Humans Language: En Journal: J Immunol Methods Year: 2022 Document type: Article Affiliation country: Germany