Epiblast inducers capture mouse trophectoderm stem cells in vitro and pattern blastoids for implantation in utero.
Cell Stem Cell
; 29(7): 1102-1118.e8, 2022 07 07.
Article
in En
| MEDLINE
| ID: mdl-35803228
The embryo instructs the allocation of cell states to spatially regulate functions. In the blastocyst, patterning of trophoblast (TR) cells ensures successful implantation and placental development. Here, we defined an optimal set of molecules secreted by the epiblast (inducers) that captures in vitro stable, highly self-renewing mouse trophectoderm stem cells (TESCs) resembling the blastocyst stage. When exposed to suboptimal inducers, these stem cells fluctuate to form interconvertible subpopulations with reduced self-renewal and facilitated differentiation, resembling peri-implantation cells, known as TR stem cells (TSCs). TESCs have enhanced capacity to form blastoids that implant more efficiently in utero due to inducers maintaining not only local TR proliferation and self-renewal, but also WNT6/7B secretion that stimulates uterine decidualization. Overall, the epiblast maintains sustained growth and decidualization potential of abutting TR cells, while, as known, distancing imposed by the blastocyst cavity differentiates TR cells for uterus adhesion, thus patterning the essential functions of implantation.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Embryo Implantation
/
Placenta
Limits:
Animals
/
Pregnancy
Language:
En
Journal:
Cell Stem Cell
Year:
2022
Document type:
Article
Affiliation country:
Austria
Country of publication:
United States