Membrane location of cardiolipin antigen in Treponema pallidum: further study on the origin of nontreponemal antibodies.
Future Microbiol
; 17: 873-886, 2022 Jul.
Article
in En
| MEDLINE
| ID: mdl-35833787
ABSTRACT
Aim:
The present study examined the membrane location of cardiolipin antigen in treponemes. Materials &methods:
The authors used different methods to disrupt the outer membrane of treponemes, detected the location of the cardiolipin antigen and analyzed the immune response in rabbits immunized with various antigens.Results:
All organisms were labeled with nontreponemal antibodies on immunoelectron and fluorescence microscopy, except the citrate buffer-treated group, which is a method leading to relatively complete removal. Except for citrate buffer-treated spirochetes, all treponemes produced low-titer, nontreponemal antibodies in immunized rabbits.Conclusion:
These findings indicated that the cardiolipin antigen was localized in the outer membrane of spirochetes. This study provided further evidence of the origin of nontreponemal antibodies during Treponema pallidum infection.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Treponema pallidum
/
Syphilis
Limits:
Animals
Language:
En
Journal:
Future Microbiol
Journal subject:
MICROBIOLOGIA
Year:
2022
Document type:
Article
Affiliation country:
China