Full Assessment of Lung Mechanics Using Computer-Controlled, Forced Oscillation Technique.
Curr Protoc
; 2(7): e488, 2022 Jul.
Article
in En
| MEDLINE
| ID: mdl-35834677
ABSTRACT
The forced oscillation technique (FOT) is a powerful and accurate method to quantify the mechanical properties of the airways and tissues of the respiratory system. Here we provide a detailed protocol for the measurement of mouse respiratory mechanical parameters. We present a procedure for mouse endotracheal intubation using a handcrafted intubation platform and confirmation module. The FlexiVentFX™ system (Scireq Inc.) is utilized for the thorough assessment of lung function with the FlexiWare™ software serving as a unit for the planning, experimentation, and analysis. The protocol has been standardized and adapted for use by our center for lung-function phenotyping of mouse models generated for the International Mouse Phenotyping Consortium (IMPC). The simplified steps, technical considerations, and integrated hardware-software demonstration make this protocol adaptable and implementable for researchers interested in using FOT for lung-function evaluation. © 2022 The Authors. Current Protocols published by Wiley Periodicals LLC. Support Protocol 1 Assembly of the FlexiVentFX™ system for measurements Support Protocol 2 FlexiWare database management Support Protocol 3 A guide for the construction of intubation platform and confirmation module Basic Protocol 1 Mouse endotracheal intubation Basic Protocol 2 Assessment of mouse basal lung function.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Respiratory Mechanics
/
Lung
Limits:
Animals
Language:
En
Journal:
Curr Protoc
Year:
2022
Document type:
Article
Affiliation country:
Czech Republic