A defined road to tracheal reconstruction: laser structuring and cell support for rapid clinic translation.
Stem Cell Res Ther
; 13(1): 317, 2022 07 16.
Article
in En
| MEDLINE
| ID: mdl-35842689
ABSTRACT
One of the severe complications occurring because of the patient's intubation is tracheal stenosis. Its incidence has significantly risen because of the COVID-19 pandemic and tends only to increase. Here, we propose an alternative to the donor trachea and synthetic prostheses-the tracheal equivalent. To form it, we applied the donor trachea samples, which were decellularized, cross-linked, and treated with laser to make wells on their surface, and inoculated them with human gingiva-derived mesenchymal stromal cells. The fabricated construct was assessed in vivo using nude (immunodeficient), immunosuppressed, and normal mice and rabbits. In comparison with the matrix ones, the tracheal equivalent samples demonstrated the thinning of the capsule, the significant vessel ingrowth into surrounding tissues, and the increase in the submucosa resorption. The developed construct was shown to be highly biocompatible and efficient in trachea restoration. These results can facilitate its clinical translation and be a base to design clinical trials.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Tissue Engineering
/
COVID-19
Limits:
Animals
/
Humans
Language:
En
Journal:
Stem Cell Res Ther
Year:
2022
Document type:
Article
Affiliation country:
RUSSIA