Molecular interactions involved in the complexation process between buffalo whey proteins concentrate and folic acid.
Food Chem
; 396: 133734, 2022 Dec 01.
Article
in En
| MEDLINE
| ID: mdl-35870246
ABSTRACT
Using buffalo whey proteins as carrier agents of sensitive molecules raises an interesting approach allowing adding value and minimizing the pollution impact of this by-product. In this context, this work aims to explore the molecular interactions between buffalo whey proteins concentrate (BWPC) and folic acid (FA). For this purpose, fluorescence, UV and FTIR analysis were performed on aqueous or solid dispersions of a buffalo whey protein concentrate (5 µM) (BWPC), with variable concentrations (0-20 µM) of FA. Fluorescence and absorption data were fitted by Stern-Volmer, Beckett, Förster resonance energy transfer, and sphere-of-action models (R2 > 0.9). Derived results suggest that BWPC strongly bind to FA through non-covalent interactions and form ground-state complexes. Additionally, BWPC improves the photostability of FA against UV radiation, and chemical denaturation negatively affects the binding properties. Obtained results encourage further studies of BWPC as carrier agents, which could promote innovative applications for this under-utilized proteins source.
Key words
Acetic acid (PubChem CID: 176); Acrylamide (PubChem CID: 6579); Buffalo whey proteins; Fluorescence spectroscopy; Folic acid; Folic acid (PubChem CID: 135398658); Photodegradation; Quenching; Sodium dodecyl sulfate (PubChem CID: 3423265); Tris-base (PubChem CID: 6503); Vitamin B9; methanol (PubChem CID: 887)
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Whey Proteins
/
Folic Acid
Type of study:
Prognostic_studies
Limits:
Animals
Language:
En
Journal:
Food Chem
Year:
2022
Document type:
Article