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Validation of a liquid chromatography coupled to tandem mass spectrometry method for simultaneous quantification of tryptophan and 10 key metabolites of the kynurenine pathway in plasma and urine: Application to a cohort of acute kidney injury patients.
Nadour, Zahia; Simian, Christophe; Laprévote, Olivier; Loriot, Marie-Anne; Larabi, Islam Amine; Pallet, Nicolas.
Affiliation
  • Nadour Z; Department of Clinical Chemistry, Georges Pompidou European Hospital, AP-HP, 20 rue Leblanc, 75015 Paris, France; Paris University, INSERM UMRS1138, Centre de Recherche des Cordeliers, 15 rue de l'Ecole de médecine, 75006 Paris, France. Electronic address: zahia.nadour@aphp.fr.
  • Simian C; Department of Clinical Chemistry, Georges Pompidou European Hospital, AP-HP, 20 rue Leblanc, 75015 Paris, France.
  • Laprévote O; Department of Clinical Chemistry, Georges Pompidou European Hospital, AP-HP, 20 rue Leblanc, 75015 Paris, France.
  • Loriot MA; Department of Clinical Chemistry, Georges Pompidou European Hospital, AP-HP, 20 rue Leblanc, 75015 Paris, France; Paris University, INSERM UMRS1138, Centre de Recherche des Cordeliers, 15 rue de l'Ecole de médecine, 75006 Paris, France.
  • Larabi IA; Department of Pharmacology and Toxicology, Paris-Saclay University (Versailles Saint-Quentin-En-Yvelines University), Inserm U-1173, Raymond Poincaré Hospital, AP-HP, 104 Boulevard Raymond Poincaré, 92380 Garches, France.
  • Pallet N; Department of Clinical Chemistry, Georges Pompidou European Hospital, AP-HP, 20 rue Leblanc, 75015 Paris, France; Paris University, INSERM UMRS1138, Centre de Recherche des Cordeliers, 15 rue de l'Ecole de médecine, 75006 Paris, France.
Clin Chim Acta ; 534: 115-127, 2022 Sep 01.
Article in En | MEDLINE | ID: mdl-35870540
ABSTRACT
A sensitive and rapid liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed and validated for the simultaneous determination of tryptophan (Trp) and ten metabolites of kynurenine pathway, including kynurenine (Kyn), 3-hydroxy-kynurenine (3-HK), kynurenic acid (KA), xanthurenic acid (XA), 3-Hydroxy-anthranilic acid (3-HANA), quinolinic acid (QA), nicotinic acid mononucleotide (NaMN), picolinic acid (Pic), nicotinamide (NAM) and nicotinic acid (NA) in both plasma and urine. This LC-MS/MS method was used to predict the occurrence of acute kidney injury (AKI) in a cohort of patients with cardiac surgery under cardiopulmonary bypass (CPB). Urinary concentrations of Pic, as well as Pic to Trp and Pic to 3-HANA ratios were highly predictive of an AKI episode the week after CPB, indicating that Pic could be a predictive biomarker of AKI. Thus, monitoring the kynurenine pathway activity with this LC-MS/MS method is a clinically relevant tool to identify new biomarkers of kidney injury.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Acute Kidney Injury / Kynurenine Type of study: Diagnostic_studies / Prognostic_studies / Risk_factors_studies Limits: Humans Language: En Journal: Clin Chim Acta Year: 2022 Document type: Article
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Acute Kidney Injury / Kynurenine Type of study: Diagnostic_studies / Prognostic_studies / Risk_factors_studies Limits: Humans Language: En Journal: Clin Chim Acta Year: 2022 Document type: Article