Use of stable isotopes to study bioconversion and bioefficacy of provitamin A carotenoids.
Methods Enzymol
; 670: 399-422, 2022.
Article
in En
| MEDLINE
| ID: mdl-35871842
ABSTRACT
Provitamin A carotenoids (pVACs) are important contributors to vitamin A status and in reducing vitamin A deficiency in vulnerable populations. However, there are uncertainties about the effectiveness of dietary pVACs to provide sufficient amounts of vitamin A due to large variations in provitamin A bioefficacy, and if provitamin A carotenoids other than ß-carotene could be utilized in biofortification programs. Although animal studies have compared the bioefficacy of ß-cryptoxanthin and ß-carotene in biofortified maize by measuring liver retinol concentrations after dietary exposure, it is unclear whether the higher bioavailability of ß-cryptoxanthin can be offset by the lower conversion of ß-cryptoxanthin to retinol, and how post-intestinal conversion of ß-cryptoxanthin may contribute to the total bioefficacy of ß-cryptoxanthin. Here, we present a method that, for the first time, uses stable isotope labeled ß-cryptoxanthin and ß-carotene to quantify bioconversion and bioefficacy of both pVACs simultaneously in human volunteers. The paper describes how positioning of the [13C] labels around the centric 15,15' double bond on either the ß-carotene or ß-cryptoxanthin molecule allows quantification of retinoids from both pVACs, and details the procedure for sample preparation and analysis using LC-MS/MS. Finally, we apply and discuss recent approaches to quantify bioconversion and bioefficacy of isotopically labeled ß-carotene and ß-cryptoxanthin in humans.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Vitamin A
/
Provitamins
Limits:
Animals
/
Humans
Language:
En
Journal:
Methods Enzymol
Year:
2022
Document type:
Article
Affiliation country:
United kingdom