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CRISPR-based systems for sensitive and rapid on-site COVID-19 diagnostics.
Soh, Jun Hui; Balleza, Enrique; Abdul Rahim, Muhammad Nadjad; Chan, Hsi-Min; Mohd Ali, Siswand; Chuah, Jacqueline Kai Chin; Edris, Sherif; Atef, Ahmed; Bahieldin, Ahmed; Ying, Jackie Y; Sabir, Jamal S M.
Affiliation
  • Soh JH; Cellbae Pte Ltd, 61 Science Park Road, The Galen, #03-07/08, Singapore 117525, Singapore.
  • Balleza E; Cellbae Pte Ltd, 61 Science Park Road, The Galen, #03-07/08, Singapore 117525, Singapore.
  • Abdul Rahim MN; Cellbae Pte Ltd, 61 Science Park Road, The Galen, #03-07/08, Singapore 117525, Singapore.
  • Chan HM; Cellbae Pte Ltd, 61 Science Park Road, The Galen, #03-07/08, Singapore 117525, Singapore.
  • Mohd Ali S; NanoBio Lab, Agency for Science, Technology and Research (A*STAR), 31 Biopolis Way, The Nanos, Singapore 138669, Singapore; A*STAR Infectious Diseases Labs, A*STAR, 31 Biopolis Way, The Nanos, Singapore 138669, Singapore.
  • Chuah JKC; Cellbae Pte Ltd, 61 Science Park Road, The Galen, #03-07/08, Singapore 117525, Singapore.
  • Edris S; Department of Biological Sciences, Faculty of Science, King Abdulaziz University, PO Box 80141, Jeddah 21589, Saudi Arabia; Centre of Excellence in Bionanoscience Research, King Abdulaziz University, Jeddah 21589, Saudi Arabia; Al-Borg Medical Laboratories, Al Borg Diagnostics, Jeddah, Saudi Arabia.
  • Atef A; Department of Biological Sciences, Faculty of Science, King Abdulaziz University, PO Box 80141, Jeddah 21589, Saudi Arabia; Centre of Excellence in Bionanoscience Research, King Abdulaziz University, Jeddah 21589, Saudi Arabia.
  • Bahieldin A; Department of Biological Sciences, Faculty of Science, King Abdulaziz University, PO Box 80141, Jeddah 21589, Saudi Arabia; Centre of Excellence in Bionanoscience Research, King Abdulaziz University, Jeddah 21589, Saudi Arabia.
  • Ying JY; NanoBio Lab, Agency for Science, Technology and Research (A*STAR), 31 Biopolis Way, The Nanos, Singapore 138669, Singapore; A*STAR Infectious Diseases Labs, A*STAR, 31 Biopolis Way, The Nanos, Singapore 138669, Singapore; Institute of Materials Research and Engineering (IMRE), A*STAR, 31 Biopolis Wa
  • Sabir JSM; Department of Biological Sciences, Faculty of Science, King Abdulaziz University, PO Box 80141, Jeddah 21589, Saudi Arabia; Centre of Excellence in Bionanoscience Research, King Abdulaziz University, Jeddah 21589, Saudi Arabia. Electronic address: jsabir@kau.edu.sa.
Trends Biotechnol ; 40(11): 1346-1360, 2022 11.
Article in En | MEDLINE | ID: mdl-35871983
ABSTRACT
The COVID-19 pandemic has strained healthcare systems. Sensitive, specific, and timely COVID-19 diagnosis is crucial for effective medical intervention and transmission control. RT-PCR is the most sensitive/specific, but requires costly equipment and trained personnel in centralized laboratories, which are inaccessible to resource-limited areas. Antigen rapid tests enable point-of-care (POC) detection but are significantly less sensitive/specific. CRISPR-Cas systems are compatible with isothermal amplification and dipstick readout, enabling sensitive/specific on-site testing. However, improvements in sensitivity and workflow complexity are needed to spur clinical adoption. We outline the mechanisms/strategies of major CRISPR-Cas systems, evaluate their on-site diagnostic capabilities, and discuss future research directions.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: COVID-19 Type of study: Diagnostic_studies Limits: Humans Language: En Journal: Trends Biotechnol Year: 2022 Document type: Article Affiliation country: Singapore
Fulltext
Add to My VHL
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: COVID-19 Type of study: Diagnostic_studies Limits: Humans Language: En Journal: Trends Biotechnol Year: 2022 Document type: Article Affiliation country: Singapore