Nanoinspired Biocompatible Chemosensors: Progress toward Efficient Prognosis of Arsenic Poisoning.
ACS Appl Bio Mater
; 5(8): 3850-3858, 2022 08 15.
Article
in En
| MEDLINE
| ID: mdl-35926152
ABSTRACT
Diagnosing heavy metals poisoning in human beings is of paramount importance. In this work, we present the design of a biocompatible FexNi(1-x)O hierarchical nanostructure-based sensor for ultraselective detection of arsenate (As(V)) ions in biological environments (e.g., body fluids, blood plasma, etc.). A novel iron doping technique was employed to fabricate the nanostructures rich with Fe cores to induce ultraselectivity toward arsenates. These nanostructures were used as dispersed markers and thin films deposited on Si/SiO2 substrates to support in vivo and in vitro detection of As(V) ions. The device demonstrated excellent sensitivity with a maximum response of 64.7% (for 1000 ppm As(V) ions) with a limit of detection of 1 ppb in blood plasma. The sensor's response time (τr) was 5 s with 95.48% recovery with a maximum error of ±0.549% after three washes. The device showed excellent response stability for 63 days with a maximum error of ±1.27%. The sensor devices were highly reproducible, with a maximum variation of ±0.6% in response for a batch of four devices. Due to Fe doping, the nanostructures in suspension demonstrated as arsenate markers with excellent cytocompatibility (with dosage up to 1 mg/mL) for human umbilical vein endothelial cells and 3T3 fibroblasts (LDH < 120 and cell viability â¼80%) till 48 h of incubation. The sensing mechanism suggested that the nanostructures not only detect arsenates but also prevent their substantial reduction to arsenites under anoxic environments. Thus, the sensors may show considerable progress toward early arsenate detection in living systems.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Arsenates
/
Arsenic Poisoning
Type of study:
Diagnostic_studies
/
Prognostic_studies
Limits:
Humans
Language:
En
Journal:
ACS Appl Bio Mater
Year:
2022
Document type:
Article
Affiliation country:
United States