An Electrochemical Sensor for Detection of Neuroblastoma Markers: Complexation Studies as a Tool for the Selection of a Suitable Receptor for Electrode Coating.
Chempluschem
; 87(8): e202200165, 2022 08.
Article
in En
| MEDLINE
| ID: mdl-35943176
ABSTRACT
Homovanillate (HVA) and vanilmandelate (VMA) are recognized markers of diseases, including neuroblastoma. However, their detection in urine represents a challenging task due to the complexity of the matrix. Here, a design, synthesis and thorough investigation of polymerizable urea-based receptors interacting with HVA and VMA are reported. The selection of receptor with the best anion recognition properties for electrode coating is based on 1 H-NMR and UV-Vis complexation studies. The sensor is prepared by electropolymerization with progress monitoring by cyclic voltammetry. The deposited layer is characterized by IR and scanning electron microscopy. The obtained sensor shows an electrochemical impedance spectroscopy response to VMA with linear range 9.9×10-6 to 1.2×10-3 â
M and LOD of 3.4×10-6 â
M. The sensor selectivity was demonstrated by the determination of VMA level in the presence of 16â
µM HVA and in artificial urine with and without phosphates, with standard deviations of 0.11, 0.17 and 0.09, respectively.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Neuroblastoma
Type of study:
Diagnostic_studies
Limits:
Humans
Language:
En
Journal:
Chempluschem
Year:
2022
Document type:
Article
Affiliation country:
Czech Republic
Publication country:
ALEMANHA
/
ALEMANIA
/
DE
/
DEUSTCHLAND
/
GERMANY