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A pendulum of induction between the epiblast and extra-embryonic endoderm supports post-implantation progression.
Vrij, Erik J; Scholte Op Reimer, Yvonne S; Roa Fuentes, Laury; Misteli Guerreiro, Isabel; Holzmann, Viktoria; Frias Aldeguer, Javier; Sestini, Giovanni; Koo, Bon-Kyoung; Kind, Jop; van Blitterswijk, Clemens A; Rivron, Nicolas C.
Affiliation
  • Vrij EJ; MERLN Institute for Technology-inspired Regenerative Medicine, Maastricht University, Universiteitssingel 40, 6229 ER Maastricht, Netherlands.
  • Scholte Op Reimer YS; Institute of Molecular Biotechnology of the Austrian Academy of Sciences, Vienna Biocenter, Dr. Bohr-Gasse 3, 1030 Vienna, Austria.
  • Roa Fuentes L; Institute of Molecular Biotechnology of the Austrian Academy of Sciences, Vienna Biocenter, Dr. Bohr-Gasse 3, 1030 Vienna, Austria.
  • Misteli Guerreiro I; MERLN Institute for Technology-inspired Regenerative Medicine, Maastricht University, Universiteitssingel 40, 6229 ER Maastricht, Netherlands.
  • Holzmann V; Hubrecht Institute, Royal Netherlands Academy of Arts and Sciences (KNAW) and University Medical Center Utrecht, UtrechtUppsalalaan 8, 3584 CT Utrecht, Netherlands.
  • Frias Aldeguer J; Institute of Molecular Biotechnology of the Austrian Academy of Sciences, Vienna Biocenter, Dr. Bohr-Gasse 3, 1030 Vienna, Austria.
  • Sestini G; MERLN Institute for Technology-inspired Regenerative Medicine, Maastricht University, Universiteitssingel 40, 6229 ER Maastricht, Netherlands.
  • Koo BK; Hubrecht Institute, Royal Netherlands Academy of Arts and Sciences (KNAW) and University Medical Center Utrecht, UtrechtUppsalalaan 8, 3584 CT Utrecht, Netherlands.
  • Kind J; Institute of Molecular Biotechnology of the Austrian Academy of Sciences, Vienna Biocenter, Dr. Bohr-Gasse 3, 1030 Vienna, Austria.
  • van Blitterswijk CA; Institute of Molecular Biotechnology of the Austrian Academy of Sciences, Vienna Biocenter, Dr. Bohr-Gasse 3, 1030 Vienna, Austria.
  • Rivron NC; Hubrecht Institute, Royal Netherlands Academy of Arts and Sciences (KNAW) and University Medical Center Utrecht, UtrechtUppsalalaan 8, 3584 CT Utrecht, Netherlands.
Development ; 149(20)2022 10 15.
Article in En | MEDLINE | ID: mdl-35993866
Embryogenesis is supported by dynamic loops of cellular interactions. Here, we create a partial mouse embryo model to elucidate the principles of epiblast (Epi) and extra-embryonic endoderm co-development (XEn). We trigger naive mouse embryonic stem cells to form a blastocyst-stage niche of Epi-like cells and XEn-like cells (3D, hydrogel free and serum free). Once established, these two lineages autonomously progress in minimal medium to form an inner pro-amniotic-like cavity surrounded by polarized Epi-like cells covered with visceral endoderm (VE)-like cells. The progression occurs through reciprocal inductions by which the Epi supports the primitive endoderm (PrE) to produce a basal lamina that subsequently regulates Epi polarization and/or cavitation, which, in return, channels the transcriptomic progression to VE. This VE then contributes to Epi bifurcation into anterior- and posterior-like states. Similarly, boosting the formation of PrE-like cells within blastoids supports developmental progression. We argue that self-organization can arise from lineage bifurcation followed by a pendulum of induction that propagates over time.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Endoderm / Germ Layers Limits: Animals Language: En Journal: Development Journal subject: BIOLOGIA / EMBRIOLOGIA Year: 2022 Document type: Article Affiliation country: Netherlands Country of publication: United kingdom

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Endoderm / Germ Layers Limits: Animals Language: En Journal: Development Journal subject: BIOLOGIA / EMBRIOLOGIA Year: 2022 Document type: Article Affiliation country: Netherlands Country of publication: United kingdom