The role of ficolin as a pattern recognition receptor in antibacterial immunity in Eriocheir sinensis.
Fish Shellfish Immunol
; 128: 494-504, 2022 Sep.
Article
in En
| MEDLINE
| ID: mdl-36002084
ABSTRACT
Ficolin, a member of the fibrinogen-related proteins family (FREPs), functions as a pattern recognition receptor (PRR) in vertebrates and in invertebrates as a novel lectin. In this study, we discovered the Ficolin homolog of Chinese mitten crab (Eriocheir sinensis), which we named EsFicolin. The obtained sequence showed that it has a highly conserved C-terminal fibrinogen-related domain (FReD) and a coiled-coil structure for trimer formation. EsFicolin was up-regulated in hemocytes after being stimulated by bacteria. Recombinant EsFicolin protein binds to gram-negative and gram-positive bacteria and agglutinates bacteria through pathogen-associated molecular patterns. In-depth study found that recombinant EsFicolin could effectively remove bacteria and showed direct antibacterial activity. EsFicolin could also promote the phagocytosis of hemocytes to enhance bacterial clearance. These findings suggest that EsFicolin plays an important role in the crab antibacterial immune response.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Brachyura
/
Pathogen-Associated Molecular Pattern Molecules
Limits:
Animals
Language:
En
Journal:
Fish Shellfish Immunol
Journal subject:
BIOLOGIA
/
MEDICINA VETERINARIA
Year:
2022
Document type:
Article
Affiliation country:
China