Effect of Conditioned Media of Limbal Epithelial Cells and Corneal Stromal Cells on Functional Activity of Limb Mesenchymal Stem Cells.
Bull Exp Biol Med
; 173(4): 464-467, 2022 Aug.
Article
in En
| MEDLINE
| ID: mdl-36058964
ABSTRACT
We studied the effect of conditioned media from limbal epithelial stem cells, fibroblasts, and corneal keratocytes on the functional activity of human limbal mesenchymal stem cells. It was shown that the conditioned media from limbal epithelial stem cells reduced proliferative activity and inhibited migration of limbal mesenchymal stem cells. In the conditioned media of limbal epithelial stem cells, increased concentrations of VEGF and TNFα and reduced concentration of BDNF, vimentin, and fibronectin were found. The conditioned medium from corneal stromal cells did not affect functional activity of mesenchymal stem cells in the limbus. These data contribute to the understanding of the interaction of cells in the limbal niche and with corneal cells essential for the maintenance of the cellular homeostasis in the cornea.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Limbus Corneae
/
Epithelium, Corneal
/
Mesenchymal Stem Cells
Limits:
Humans
Language:
En
Journal:
Bull Exp Biol Med
Year:
2022
Document type:
Article
Affiliation country:
RUSSIA