Your browser doesn't support javascript.
loading
Generation of two induced pluripotent stem cell lines (RCMGi005-A/B) from human skin fibroblasts of a cystic fibrosis patient with homozygous F508del mutation in CFTR gene.
Panchuk, Irina; Kondrateva, Ekaterina; Demchenko, Anna; Grigorieva, Olga; Erofeeva, Anastasija; Amelina, Elena; Tabakov, Vyacheslav; Orlova, Maria; Voronina, Ekaterina; Pozhitnova, Victoria; Lavrov, Alexander; Smirnikhina, Svetlana; Kutsev, Sergey.
Affiliation
  • Panchuk I; Research Centre for Medical Genetics, Moscow, Russian Federation. Electronic address: irina.panchuk42@gmail.com.
  • Kondrateva E; Research Centre for Medical Genetics, Moscow, Russian Federation.
  • Demchenko A; Research Centre for Medical Genetics, Moscow, Russian Federation.
  • Grigorieva O; Research Centre for Medical Genetics, Moscow, Russian Federation.
  • Erofeeva A; Research Centre for Medical Genetics, Moscow, Russian Federation.
  • Amelina E; The Research Institute of Pulmonology, Moscow 115682, Russian Federation.
  • Tabakov V; Research Centre for Medical Genetics, Moscow, Russian Federation.
  • Orlova M; Research Centre for Medical Genetics, Moscow, Russian Federation.
  • Voronina E; Research Centre for Medical Genetics, Moscow, Russian Federation.
  • Pozhitnova V; Research Centre for Medical Genetics, Moscow, Russian Federation.
  • Lavrov A; Research Centre for Medical Genetics, Moscow, Russian Federation.
  • Smirnikhina S; Research Centre for Medical Genetics, Moscow, Russian Federation.
  • Kutsev S; Research Centre for Medical Genetics, Moscow, Russian Federation.
Stem Cell Res ; 64: 102896, 2022 10.
Article in En | MEDLINE | ID: mdl-36067639
Induced pluripotent stem cells (iPSCs) was successfully generated from skin fibroblast obtained from patient with cystic fibrosis by using non-integrating, viral CytoTune™-iPS 2.0 Sendai Reprogramming Kit, which contain three vectors preparation: polycistronic Klf4-Oct3/4-Sox2, cMyc, and Klf4. Created iPSC lines showed a normal karyotype, expressed pluripotency markers and demonstrated the potential to differentiate into three germ layers in spontaneous differentiation assay.
Subject(s)
Key words
Fulltext
Add to My VHL
Print
XML
PubMed Links
Search on Google

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Cystic Fibrosis / Induced Pluripotent Stem Cells Limits: Humans Language: En Journal: Stem Cell Res Year: 2022 Document type: Article Country of publication: United kingdom
Fulltext
Add to My VHL
Print
XML
PubMed Links
Search on Google

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Cystic Fibrosis / Induced Pluripotent Stem Cells Limits: Humans Language: En Journal: Stem Cell Res Year: 2022 Document type: Article Country of publication: United kingdom