Your browser doesn't support javascript.
loading
Engineering Shewanella oneidensis to efficiently harvest electricity power by co-utilizing glucose and lactate in thin stillage of liquor industry.
Zhang, Junqi; Wu, Deguang; Zhao, Yakun; Liu, Dingyuan; Guo, Xuewu; Chen, Yefu; Zhang, Cuiying; Sun, Xi; Guo, Ju; Yuan, Dezhi; Xiao, Dongguang; Li, Feng; Song, Hao.
Affiliation
  • Zhang J; School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China; Frontiers Science Center for Synthetic Biology (Ministry of Education), Key Laboratory of Systems Bioengineering, Tianjin University, Tianjin 300072, PR China; Synthetic Biology Research Platform, Collaborative
  • Wu D; Key Laboratory of Industrial Fermentation Microbiology (Ministry of Education), Tianjin Industrial Microbiology Key Lab, College of Biotechnology, Tianjin University of Science and Technology, Box 08, No. 29, 13ST. TEDA, Tianjin 300457, PR China; Department of Brewing Engineering, Moutai Institute,
  • Zhao Y; School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China; Synthetic Biology Research Platform, Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China; Qing
  • Liu D; School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China; Frontiers Science Center for Synthetic Biology (Ministry of Education), Key Laboratory of Systems Bioengineering, Tianjin University, Tianjin 300072, PR China; Synthetic Biology Research Platform, Collaborative
  • Guo X; Key Laboratory of Industrial Fermentation Microbiology (Ministry of Education), Tianjin Industrial Microbiology Key Lab, College of Biotechnology, Tianjin University of Science and Technology, Box 08, No. 29, 13ST. TEDA, Tianjin 300457, PR China.
  • Chen Y; Key Laboratory of Industrial Fermentation Microbiology (Ministry of Education), Tianjin Industrial Microbiology Key Lab, College of Biotechnology, Tianjin University of Science and Technology, Box 08, No. 29, 13ST. TEDA, Tianjin 300457, PR China.
  • Zhang C; Key Laboratory of Industrial Fermentation Microbiology (Ministry of Education), Tianjin Industrial Microbiology Key Lab, College of Biotechnology, Tianjin University of Science and Technology, Box 08, No. 29, 13ST. TEDA, Tianjin 300457, PR China.
  • Sun X; College of Biological Engineering, Tianjin Agricultural University, Tianjin, PR China.
  • Guo J; Department of Brewing Engineering, Moutai Institute, Luban Ave, Renhuai 564507, Guizhou, PR China.
  • Yuan D; Department of Brewing Engineering, Moutai Institute, Luban Ave, Renhuai 564507, Guizhou, PR China.
  • Xiao D; Key Laboratory of Industrial Fermentation Microbiology (Ministry of Education), Tianjin Industrial Microbiology Key Lab, College of Biotechnology, Tianjin University of Science and Technology, Box 08, No. 29, 13ST. TEDA, Tianjin 300457, PR China.
  • Li F; School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China. Electronic address: messilifeng@163.com.
  • Song H; Frontiers Science Center for Synthetic Biology (Ministry of Education), Key Laboratory of Systems Bioengineering, Tianjin University, Tianjin 300072, PR China; Synthetic Biology Research Platform, Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), School of Chemical Engin
Sci Total Environ ; 855: 158696, 2023 Jan 10.
Article in En | MEDLINE | ID: mdl-36108833
ABSTRACT
Thin stillage, rich in glucose and lactate, can seriously pollute water resources when directly discharged into the natural environment. Microbial fuel cells (MFC), as a green and sustainable technology, could utilize exoelectrogens to break down organics in wastewater and harvest electricity. Nevertheless, Shewanella oneidensis MR-1, cannot utilize thin stillage for efficient power generation. Here, to enable S. oneidensis to co-utilize glucose and lactate from thin stillage, an engineered S. oneidensis G7∆RSL1 was first created by constructing glucose metabolism pathway, promoting glucose and lactate co-utilization, and enhancing biofilm formation. Then, to enhance biofilm conductivity, we constructed a 3D self-assembled G7∆RSL1-rGO/CNT biohybrid with maximum power density of 560.4 mW m-2 and 373.7 mW m-2 in artificial and actual thin stillage, respectively, the highest among the reported genetically engineered S. oneidensis with thin stillage as carbon source. This study provides a new strategy to facilitate practical applications of MFC in wastewater remediation and efficient power recovery.
Subject(s)
Key words

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Bioelectric Energy Sources / Shewanella Language: En Journal: Sci Total Environ Year: 2023 Document type: Article

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Bioelectric Energy Sources / Shewanella Language: En Journal: Sci Total Environ Year: 2023 Document type: Article