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Acyloxyacyl hydrolase deficiency induces chronic inflammation and bone loss in male mice.
Cheng, Xu; Song, Xiaoting; Li, Zhiyan; Yuan, Chiting; Lei, Xinhuan; Feng, Mingxuan; Hong, Zhenghua; Zhang, Liwei; Hong, Dun.
Affiliation
  • Cheng X; Department of Orthopedics, Taizhou Hospital Affiliated to Wenzhou Medical University, Linhai, China.
  • Song X; Enze Medical Center, Taizhou, China.
  • Li Z; Bone Metabolism and Development Research Center, Taizhou Hospital Affiliated to Wenzhou Medical University, Linhai, China.
  • Yuan C; Department of Orthopedics, Taizhou Hospital Affiliated to Wenzhou Medical University, Linhai, China.
  • Lei X; Enze Medical Center, Taizhou, China.
  • Feng M; Bone Metabolism and Development Research Center, Taizhou Hospital Affiliated to Wenzhou Medical University, Linhai, China.
  • Hong Z; Department of Orthopedics, Taizhou Hospital Affiliated to Wenzhou Medical University, Linhai, China.
  • Zhang L; Enze Medical Center, Taizhou, China.
  • Hong D; Bone Metabolism and Development Research Center, Taizhou Hospital Affiliated to Wenzhou Medical University, Linhai, China.
J Mol Med (Berl) ; 100(11): 1599-1616, 2022 11.
Article in En | MEDLINE | ID: mdl-36112153
ABSTRACT
Hormonal homeostasis is essential in bone remodeling. Recent studies have shown that the treatment of intestinal inflammation can result in the regulation of bone resorption in distant bones. Increased intestinal permeability may lead to systemic inflammation and bone loss, also known as gut-bone axis. However, the underlying mechanism remains to be elucidated. Lipopolysaccharide (LPS) is a component of gram-negative bacteria that can increase osteoclastic differentiation in vitro. Acyloxyacyl hydrolase (AOAH) is a specific degrading enzyme of LPS, but little is known about the role of AOAH in bone metabolism. In this study, adult Aoah-/- mice showed a chronic inflammatory state and osteopenic phenotype analyzed by micro-CT and HE staining. Tartrate-resistant acid phosphatase (TRAP) staining of femurs showed an increase in TRAP-positive cells from Aoah-/- mice. AOAH depletion enhanced the osteoclast differentiation and bone resorption capacity of bone marrow-derived macrophages (BMMs). The enhanced osteoclast differentiation and bone resorption capacity of Aoah-/- BMMs were reversed by rAOAH. In conclusion, the chronic inflammatory state of adult Aoah-/- mice promotes bone resorption. AOAH participates in bone metabolism, which is mainly mediated by inhibiting osteoclast differentiation. LPS may be a key mediator of the gut-bone axis, and targeting AOAH may represent a feasible strategy for the treatment of chronic inflammatory bone resorption. KEY MESSAGES AOAH knockout mice exhibited chronic inflammation mediated by LPS, and LPS may also serve as an important mediator in the regulation of bone metabolism in the gut-bone axis. AOAH regulated bone resorption by blocking the osteoclast differentiation via classical ERK and JNK pathways. rAOAH could rescue the enhanced osteoclast differentiation caused by AOAH deficiency.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Bone Resorption / Lipopolysaccharides Limits: Animals Language: En Journal: J Mol Med (Berl) Journal subject: BIOLOGIA MOLECULAR / GENETICA MEDICA Year: 2022 Document type: Article Affiliation country: China

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Bone Resorption / Lipopolysaccharides Limits: Animals Language: En Journal: J Mol Med (Berl) Journal subject: BIOLOGIA MOLECULAR / GENETICA MEDICA Year: 2022 Document type: Article Affiliation country: China
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