The role of voltage-gated sodium channel genotypes in pyrethroid resistance in Aedes aegypti in Taiwan.
PLoS Negl Trop Dis
; 16(9): e0010780, 2022 09.
Article
in En
| MEDLINE
| ID: mdl-36137080
ABSTRACT
BACKGROUND:
Aedes aegypti is the major vector of dengue that threatens public health in tropical and subtropical regions. Pyrethroid-based control strategies effectively control this vector, but the repeated usage of the same insecticides leads to resistance and hampers control efforts. Therefore, efficient and prompt monitoring of insecticide resistance in local mosquito populations is critical for dengue control. METHODOLOGY/PRINCIPALFINDING:
We collected Ae. aegypti in southern Taiwan in March and October 2016. We analyzed the voltage-gated sodium channel (vgsc) genotypes of parentals (G0) and G1 adults after cypermethrin insecticide bioassay. Our results showed that four VGSC mutations (S989P, V1016G, F1534C, and D1763Y) associated with resistance were commonly detected in field-collected Ae. aegypti. The frequencies of these four mutations in the local mosquito population were significantly higher in October (0.29, 0.4, 0.27 and 0.11) than in March (0.09, 0.16, 0.18 and 0.03). Specific vgsc combined genotypes composed of the one to four such mutations (SGFY/SGFY, SVCD/SVCD, SGFY/PGFD, SVCD/SGFY, PGFD/PGFD, and SVCD/PGFD) shifted towards higher frequencies in October, implying their resistance role. In addition, the cypermethrin exposure bioassay data supported the field observations. Moreover, our study observed an association between the resistance level and the proportion of resistance genotypes in the population. CONCLUSIONS/SIGNIFICANCE:
This is the first study to demonstrate the role of four-locus vgsc genotypes in resistance evaluation in a local Ae. aegypti population in Taiwan. This alternative method using resistance-associated genotypes as an indicator of practically insecticide resistance monitoring is a useful tool for providing precise and real-time information for decision makers.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Pyrethrins
/
Aedes
/
Dengue
/
Voltage-Gated Sodium Channels
/
Insecticides
Type of study:
Prognostic_studies
Limits:
Animals
/
Humans
Country/Region as subject:
Asia
Language:
En
Journal:
PLoS Negl Trop Dis
Journal subject:
MEDICINA TROPICAL
Year:
2022
Document type:
Article
Affiliation country:
Taiwan