A dispersive liquid-liquid microextraction followed by reverse-phase high-performance liquid chromatography for QuEChERS determination of chlorogenic acid.
Phytochem Anal
; 34(1): 30-39, 2023 Jan.
Article
in En
| MEDLINE
| ID: mdl-36151736
ABSTRACT
INTRODUCTION:
The presence of chlorogenic acid (CGA) not only imparts a characteristic flavour to foods but also makes them useful against chronic diseases and metabolic syndromes, especially diabetes mellitus and asthma.OBJECTIVES:
The present study was designed to develop a quick, easy, cheap, effective, rugged, and safe (QuEChERS) and pragmatic method to analyse CGA in plant-based products by applying dispersive liquid-liquid microextraction (DLLME) followed by reverse-phase high-performance liquid chromatography under a diode array detector (RP-HPLC-DAD).METHODOLOGY:
The DLLME was carried out using Triton X-100 as a dispersant and ethanol as an extraction solvent, while method development and validation activities were performed on a Shimadzu 10-AT HPLC-DAD system equipped with C-18 columns as stationary phases.RESULTS:
The well-resolved peak for the standard CGA was observed at 2.92 min for the mobile phase comprising 0.1% aqueous formic acid-ethanol (2278 v/v) of pH 3.0 programmed under an isocratic flow rate of 1.0 mL/min. The developed method was found to be linear (R2 = 0.9976) over 1-500 µg/mL of CGA concentration at 328 nm. Moreover, the assay was found to be repeatable with RSD ≤ 5.0, and the limit of detection (LOD) and limit of quantification (LOQ) were 0.0281 and 0.0853 µg/mL of CGA, respectively. The DLLME offered an overall recovery rate between 97.60% and 99.54% at an acceptable level of reproducibility [relative standard deviation (RSD) ≤ 5.0].CONCLUSION:
The developed assay was found to be a QuEChERS and pragmatic choice for the high-throughput analysis of CGA in plant-based products/foods. Finally, the analysis revealed the presence of an ample level of CGA in guava fruit in addition to coffee beans and black tea.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Chlorogenic Acid
/
Liquid Phase Microextraction
Language:
En
Journal:
Phytochem Anal
Journal subject:
BOTANICA
/
QUIMICA
Year:
2023
Document type:
Article
Affiliation country:
Pakistan