Metabolomics Analysis of Aqueous Humor Based on Liquid Chromatography-Mass Spectrometry.
Methods Mol Biol
; 2571: 149-155, 2023.
Article
in En
| MEDLINE
| ID: mdl-36152159
ABSTRACT
Aqueous humor (AH) is a transparent fluid that fills the anterior segment of the eye. The composition and level of metabolites in AH are important for understanding its physiology and changes caused by the occurrence of eye disease. A simple method for the preparation and analysis of AH samples was developed using the liquid chromatography-quadrupole time-of-flight mass spectrometry (LC-QTOF-MS) technique. The analyses were performed using two types of chromatography reversed-phase liquid chromatography-mass spectrometry (LC-RP-MS) and hydrophilic interaction liquid chromatography-mass spectrometry (LC-HILIC-MS), in the sample prepared by one protocol.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Aqueous Humor
/
Metabolomics
Language:
En
Journal:
Methods Mol Biol
Journal subject:
BIOLOGIA MOLECULAR
Year:
2023
Document type:
Article
Affiliation country:
Poland