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An open-source computational tool for measuring bacterial biofilm morphology and growth kinetics upon one-sided exposure to an antimicrobial source.
Gingichashvili, Sarah; Steinberg, Doron; Sionov, Ronit Vogt; Feuerstein, Osnat; Cohen, Noa E.
Affiliation
  • Gingichashvili S; The Biofilm Research Laboratory, Institute of Biomedical and Oral Research, The Faculty of Dental Medicine, The Hebrew University of Jerusalem, Jerusalem, Israel.
  • Steinberg D; The Biofilm Research Laboratory, Institute of Biomedical and Oral Research, The Faculty of Dental Medicine, The Hebrew University of Jerusalem, Jerusalem, Israel.
  • Sionov RV; The Biofilm Research Laboratory, Institute of Biomedical and Oral Research, The Faculty of Dental Medicine, The Hebrew University of Jerusalem, Jerusalem, Israel.
  • Feuerstein O; Department of Prosthodontics, Hadassah Medical Center, Faculty of Dental Medicine, The Hebrew University of Jerusalem, Jerusalem, Israel.
  • Cohen NE; School of Software Engineering and Computer Science, Azrieli College of Engineering, Jerusalem, Israel. noace@jce.ac.il.
Sci Rep ; 12(1): 16125, 2022 09 27.
Article in En | MEDLINE | ID: mdl-36167741
Bacillus subtilis biofilms are well known for their complex and highly adaptive morphology. Indeed, their phenotypical diversity and intra-biofilm heterogeneity make this gram-positive bacterium the subject of many scientific papers on the structure of biofilms. The "robustness" of biofilms is a term often used to describe their level of susceptibility to antimicrobial agents and various mechanical and molecular inhibition/eradication methods. In this paper, we use computational analytics to quantify Bacillus subtilis morphological response to proximity to an antimicrobial source, in the form of the antiseptic chlorhexidine. Chlorhexidine droplets, placed in proximity to Bacillus subtilis macrocolonies at different distances result in morphological changes, quantified using Python-based code, which we have made publicly available. Our results quantify peripheral and inner core deformation as well as differences in cellular viability of the two regions. The results reveal that the inner core, which is often characterized by the presence of wrinkled formations in the macrocolony, is more preserved than the periphery. Furthermore, the paper describes a crescent-shaped colony morphology which occurs when the distance from the chlorhexidine source is 0.5 cm, as well as changes observed in the growth substrate of macrocolonies exposed to chlorhexidine.
Subject(s)

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Anti-Infective Agents / Anti-Infective Agents, Local Language: En Journal: Sci Rep Year: 2022 Document type: Article Affiliation country: Israel Country of publication: United kingdom

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Anti-Infective Agents / Anti-Infective Agents, Local Language: En Journal: Sci Rep Year: 2022 Document type: Article Affiliation country: Israel Country of publication: United kingdom