Structural determination and pro-angiogenic effect of polysaccharide from the pollen of Typha angustifolia L.
Int J Biol Macromol
; 222(Pt B): 2028-2040, 2022 Dec 01.
Article
in En
| MEDLINE
| ID: mdl-36209909
ABSTRACT
Four fractions of polysaccharides (TPP-1, TPP-2, TPP-3, and TPP-4) were isolated and purified from the pollen of Typha angustifolia L., and the structure of TPP-3 was furtherly determined by HPGPC (High Performance Gel Permeation Chromatography), monosaccharide composition analysis, methylation analysis and NMR (Nuclear Magnetic Resonance). TPP-3 was found to be a homogeneous heteropolysaccharide with an average molecular weight of 5.5 × 104 Da and composed of eight types of monosaccharides. The pro-angiogenic activities of TPP-3 were verified on HUVECs and VEGFR tyrosine kinase inhibitor II (VRI)-induced vascular defect zebrafish model. Furthermore, the underlying mechanism investigation showed that its pro-angiogenic activities were closely related with the activation of VEGF/PI3K/Akt signaling pathway.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Zebrafish
/
Typhaceae
Type of study:
Prognostic_studies
Limits:
Animals
Language:
En
Journal:
Int J Biol Macromol
Year:
2022
Document type:
Article