Electroneutral Polymer Nanodiscs Enable Interference-Free Probing of Membrane Proteins in a Lipid-Bilayer Environment.
Small
; 18(47): e2202492, 2022 11.
Article
in En
| MEDLINE
| ID: mdl-36228092
ABSTRACT
Membrane proteins can be examined in near-native lipid-bilayer environments with the advent of polymer-encapsulated nanodiscs. These nanodiscs self-assemble directly from cellular membranes, allowing in vitro probing of membrane proteins with techniques that have previously been restricted to soluble or detergent-solubilized proteins. Often, however, the high charge densities of existing polymers obstruct bioanalytical and preparative techniques. Thus, the authors aim to fabricate electroneutral-yet water-soluble-polymer nanodiscs. By attaching a sulfobetaine group to the commercial polymers DIBMA and SMA(21), these polyanionic polymers are converted to the electroneutral maleimide derivatives, Sulfo-DIBMA and Sulfo-SMA(21). Sulfo-DIBMA and Sulfo-SMA(21) readily extract proteins and phospholipids from artificial and cellular membranes to form nanodiscs. Crucially, the electroneutral nanodiscs avert unspecific interactions, thereby enabling new insights into protein-lipid interactions through lab-on-a-chip detection and in vitro translation of membrane proteins. Finally, the authors create a library comprising thousands of human membrane proteins and use proteome profiling by mass spectrometry to show that protein complexes are preserved in electroneutral nanodiscs.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Nanostructures
/
Lipid Bilayers
Limits:
Humans
Language:
En
Journal:
Small
Journal subject:
ENGENHARIA BIOMEDICA
Year:
2022
Document type:
Article
Affiliation country:
Germany