Towards stimulated Raman scattering spectro-microscopy across the entire Raman active region using a multiple-plate continuum.
Opt Express
; 30(21): 38975-38984, 2022 Oct 10.
Article
in En
| MEDLINE
| ID: mdl-36258449
ABSTRACT
Stimulated Raman scattering (SRS) has attracted increasing attention in bio-imaging because of the ability toward background-free molecular-specific acquisitions without fluorescence labeling. Nevertheless, the corresponding sensitivity and specificity remain far behind those of fluorescence techniques. Here, we demonstrate SRS spectro-microscopy driven by a multiple-plate continuum (MPC), whose octave-spanning bandwidth (600-1300â
nm) and high spectral energy density (â¼1 nJ/cm-1) enable spectroscopic interrogation across the entire Raman active region (0-4000â
cm-1), SRS imaging of a Drosophila brain, and electronic pre-resonance (EPR) detection of a fluorescent dye. We envision that utilizing MPC light source will substantially enhance the sensitivity and specificity of SRS by implementing EPR mode and spectral multiplexing via accessing three or more coherent wavelengths.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Spectrum Analysis, Raman
/
Microscopy
Language:
En
Journal:
Opt Express
Journal subject:
OFTALMOLOGIA
Year:
2022
Document type:
Article