Detection of intestinal parasites in human faecal samples using dissolved air flotation.
Trop Med Int Health
; 27(12): 1044-1052, 2022 12.
Article
in En
| MEDLINE
| ID: mdl-36261920
ABSTRACT
OBJECTIVE:
Ova and parasite (O&P) examination is recommended for the laboratory diagnosis of agents causing parasitic infections; however, this exam requires scientific and technological improvements to enhance its diagnostic validity. Dissolved air flotation (DAF) is an efficient technical principle separating suspended solids in a liquid medium. We aimed to develop and validate a new procedure for intestinal parasite detection with DAF.METHODS:
In this study, we collected samples from 500 volunteers, screened them by direct examination, and transferred the material to tubes using the Three Faecal Test (TF-Test) for triplicate DAF tests. We evaluated physical-chemical parameters and DAF prototype components through quantifying parasites recovered from floated and non-floated regions of the flotation column. The DAF operation protocol was validated with the gold standard results.RESULTS:
The 10% saturated volume proportion and cationic surfactant showed regularity and high parasite recovery (80%). Modifications of the needle device did not influence parasite recovery (p > 0.05). Sensitivity, specificity, accuracy and kappa agreement obtained with the DAF protocol were 91%, 100%, 93% and substantial (k = 0.64), respectively.CONCLUSION:
The DAF principle could be used to process faecal samples in routine laboratory exams, enabling intestinal parasite detection.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Parasites
/
Intestinal Diseases, Parasitic
Type of study:
Diagnostic_studies
/
Guideline
Limits:
Animals
/
Humans
Language:
En
Journal:
Trop Med Int Health
Journal subject:
MEDICINA TROPICAL
/
SAUDE PUBLICA
Year:
2022
Document type:
Article
Affiliation country:
Brazil