Closed-loop optogenetic control of the dynamics of neural activity in non-human primates.
Nat Biomed Eng
; 7(4): 559-575, 2023 04.
Article
in En
| MEDLINE
| ID: mdl-36266536
ABSTRACT
Electrical neurostimulation is effective in the treatment of neurological disorders, but associated recording artefacts generally limit its applications to open-loop stimuli. Real-time and continuous closed-loop control of brain activity can, however, be achieved by pairing concurrent electrical recordings and optogenetics. Here we show that closed-loop optogenetic stimulation with excitatory opsins enables the precise manipulation of neural dynamics in brain slices from transgenic mice and in anaesthetized non-human primates. The approach generates oscillations in quiescent tissue, enhances or suppresses endogenous patterns in active tissue and modulates seizure-like bursts elicited by the convulsant 4-aminopyridine. A nonlinear model of the phase-dependent effects of optical stimulation reproduced the modulation of cycles of local-field potentials associated with seizure oscillations, as evidenced by the systematic changes in the variability and entropy of the phase-space trajectories of seizures, which correlated with changes in their duration and intensity. We also show that closed-loop optogenetic neurostimulation could be delivered using intracortical optrodes incorporating light-emitting diodes. Closed-loop optogenetic approaches may be translatable to therapeutic applications in humans.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Seizures
/
Optogenetics
Limits:
Animals
Language:
En
Journal:
Nat Biomed Eng
Year:
2023
Document type:
Article
Affiliation country:
United kingdom