Rapid and efficient room-temperature serial synchrotron crystallography using the CFEL TapeDrive.
IUCrJ
; 9(Pt 6): 778-791, 2022 Nov 01.
Article
in En
| MEDLINE
| ID: mdl-36381150
ABSTRACT
Serial crystallography at conventional synchrotron light sources (SSX) offers the possibility to routinely collect data at room temperature using micrometre-sized crystals of biological macromolecules. However, SSX data collection is not yet as routine and currently takes significantly longer than the standard rotation series cryo-crystallography. Thus, its use for high-throughput approaches, such as fragment-based drug screening, where the possibility to measure at physio-logical temperatures would be a great benefit, is impaired. On the way to high-throughput SSX using a conveyor belt based sample delivery system - the CFEL TapeDrive - with three different proteins of biological relevance (Klebsiella pneumoniae CTX-M-14 ß-lactamase, Nectria haematococca xylanase GH11 and Aspergillus flavus urate oxidase), it is shown here that complete datasets can be collected in less than a minute and only minimal amounts of sample are required.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Language:
En
Journal:
IUCrJ
Year:
2022
Document type:
Article
Affiliation country:
Germany