De novo methylation of histone H3K23 by the methyltransferases EHMT1/GLP and EHMT2/G9a.
Epigenetics Chromatin
; 15(1): 36, 2022 11 21.
Article
in En
| MEDLINE
| ID: mdl-36411491
ABSTRACT
Epigenetic modifications to histone proteins serve an important role in regulating permissive and repressive chromatin states, but despite the identification of many histone PTMs and their perceived role, the epigenetic writers responsible for generating these chromatin signatures are not fully characterized. Here, we report that the canonical histone H3K9 methyltransferases EHMT1/GLP and EHMT2/G9a are capable of catalyzing methylation of histone H3 lysine 23 (H3K23). Our data show that while both enzymes can mono- and di-methylate H3K23, only EHMT1/GLP can tri-methylate H3K23. We also show that pharmacologic inhibition or genetic ablation of EHMT1/GLP and/or EHMT2/G9a leads to decreased H3K23 methylation in mammalian cells. Taken together, this work identifies H3K23 as a new direct methylation target of EHMT1/GLP and EHMT2/G9a, and highlights the differential activity of these enzymes on H3K23 as a substrate.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Histones
/
Histone-Lysine N-Methyltransferase
Limits:
Animals
Language:
En
Journal:
Epigenetics Chromatin
Year:
2022
Document type:
Article
Affiliation country:
United States