AlphaPulldown-a python package for protein-protein interaction screens using AlphaFold-Multimer.

ABSTRACT

SUMMARY: The artificial intelligence-based structure prediction program AlphaFold-Multimer enabled structural modelling of protein complexes with unprecedented accuracy. Increasingly, AlphaFold-Multimer is also used to discover new protein-protein interactions (PPIs). Here, we present AlphaPulldown, a Python package that streamlines PPI screens and high-throughput modelling of higher-order oligomers using AlphaFold-Multimer. It provides a convenient command-line interface, a variety of confidence scores and a graphical analysis tool. AVAILABILITY AND IMPLEMENTATION AlphaPulldown is freely available at https//www.embl-hamburg.de/AlphaPulldown. SUPPLEMENTARY INFORMATION Supplementary note is available at Bioinformatics online.