Discovery of Efrotomycin Congeners and Heterologous Expression-Based Insights into the Self-Resistance Mechanism.
J Nat Prod
; 85(12): 2865-2872, 2022 12 23.
Article
in En
| MEDLINE
| ID: mdl-36445346
ABSTRACT
Four new efrotomycins, A1-A4 (1-4), were isolated from the salt mine-derived Amycolatopsis cihanbeyliensis DSM 45679 and structurally determined. Efrotomycins A3 (3) and A4 (4) feature a tetrahydrofuran ring configured distinctly from known elfamycins. Heterologous expression of the efrotomycin gene cluster (efr BGC) in Streptomyces lividans SBT18 led to efrotomycin B1 (5), the yield of which was improved several fold upon introduction of the transporter gene efrT, a putative self-resistance determinant outside of the efr BGC.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Actinomycetales
Language:
En
Journal:
J Nat Prod
Year:
2022
Document type:
Article
Affiliation country:
China