Development of a Cyclic Voltammetry-Based Method for the Detection of Antigens and Antibodies as a Novel Strategy for Syphilis Diagnosis.
Int J Environ Res Public Health
; 19(23)2022 Dec 03.
Article
in En
| MEDLINE
| ID: mdl-36498280
ABSTRACT
The improvement of laboratory diagnosis is a critical step for the reduction of syphilis cases around the world. In this paper, we present the development of an impedance-based method for detecting T. pallidum antigens and antibodies as an auxiliary tool for syphilis laboratory diagnosis. We evaluate the voltammetric signal obtained after incubation in carbon or gold nanoparticle-modified carbon electrodes in the presence or absence of Poly-L-Lysine. Our results indicate that the signal obtained from the electrodes was sufficient to distinguish between infected and non-infected samples immediately (T0') or 15 min (T15') after incubation, indicating its potential use as a point-of-care method as a screening strategy.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Syphilis
/
Metal Nanoparticles
Type of study:
Diagnostic_studies
Limits:
Humans
Language:
En
Journal:
Int J Environ Res Public Health
Year:
2022
Document type:
Article
Affiliation country:
Brazil