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Development of multiplex RT-ddPCR assays for detection of SARS-CoV-2 and other common respiratory virus infections.
Leong, Nathaniel K C; Gu, Haogao; Ng, Daisy Y M; Chang, Lydia D J; Krishnan, Pavithra; Cheng, Samuel S M; Peiris, Malik; Poon, Leo L M.
Affiliation
  • Leong NKC; School of Public Health, LKS Faculty of Medicine, The University of Hong Kong, Hong Kong.
  • Gu H; School of Public Health, LKS Faculty of Medicine, The University of Hong Kong, Hong Kong.
  • Ng DYM; School of Public Health, LKS Faculty of Medicine, The University of Hong Kong, Hong Kong.
  • Chang LDJ; School of Public Health, LKS Faculty of Medicine, The University of Hong Kong, Hong Kong.
  • Krishnan P; School of Public Health, LKS Faculty of Medicine, The University of Hong Kong, Hong Kong.
  • Cheng SSM; School of Public Health, LKS Faculty of Medicine, The University of Hong Kong, Hong Kong.
  • Peiris M; School of Public Health, LKS Faculty of Medicine, The University of Hong Kong, Hong Kong.
  • Poon LLM; Centre for Immunology and Infection, Hong Kong Science and Technology Park, Hong Kong.
Influenza Other Respir Viruses ; 17(1): e13084, 2023 01.
Article in En | MEDLINE | ID: mdl-36517993
BACKGROUND: Measures for mitigation of Coronavirus Disease 2019 (COVID-19) were set to reduce the spread of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2). SARS-CoV-2 and other respiratory viruses share similar transmission routes and some common clinical manifestations. Co-circulation of SARS-CoV-2 and other common respiratory viruses is imminent. Therefore, development of multiplex assays for detecting these respiratory viruses is essential for being prepared for future outbreaks of respiratory viruses. METHODS: A panel of three reverse transcription droplet digital PCR (RT-ddPCR) assays were developed to detect 15 different human respiratory viruses. Evaluations of its performance were demonstrated. A total of 100 local and 98 imported COVID-19 cases in Hong Kong were screened for co-infection with other common respiratory viruses. RESULTS: All detected viral targets showed distinct signal clusters using the multiplex RT-ddPCR assays. These assays have a broad range of linearity and good intra-/inter-assay reproducibility for each target. The lower limits of quantification for all targets were ≤46 copies per reaction. Six imported cases of COVID-19 were found to be co-infected with other respiratory viruses, whereas no local case of co-infection was observed. CONCLUSIONS: The multiplex RT-ddPCR assays were demonstrated to be useful for screening of respiratory virus co-infections. The strict preventive measures applied in Hong Kong may be effective in limiting the circulation of other human respiratory viruses. The multiplex assays developed in this study can achieve a robust detection method for clinical and research purposes.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Coinfection / COVID-19 Type of study: Diagnostic_studies Limits: Humans Language: En Journal: Influenza Other Respir Viruses Journal subject: VIROLOGIA Year: 2023 Document type: Article Affiliation country: Hong Kong Country of publication: United kingdom

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Coinfection / COVID-19 Type of study: Diagnostic_studies Limits: Humans Language: En Journal: Influenza Other Respir Viruses Journal subject: VIROLOGIA Year: 2023 Document type: Article Affiliation country: Hong Kong Country of publication: United kingdom