Extraction and Oxford Nanopore sequencing of genomic DNA from filamentous Actinobacteria.
STAR Protoc
; 4(1): 101955, 2023 03 17.
Article
in En
| MEDLINE
| ID: mdl-36527715
ABSTRACT
Actinomycetota (Actinobacteria) is an ecologically and industrially important phylum which is challenging to extract pure high-molecular-weight (HMW) DNA from. This protocol provides a parallelized, cost-effective, and straightforward approach for consistently extracting pure HMW DNA using modified non-toxic commercial kits suitable for higher throughput applications. We further provide a workflow for sequencing and assembly of complete genomes using an optimized Oxford Nanopore rapid barcoding protocol and Illumina data error correction.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Actinobacteria
/
Nanopore Sequencing
Language:
En
Journal:
STAR Protoc
Year:
2023
Document type:
Article
Affiliation country:
Denmark