Unusual ciliate-specific codons in Tetrahymena mRNAs are translated correctly in a rabbit reticulocyte lysate supplemented with a subcellular fraction from Tetrahymena.
Biochem J
; 244(2): 331-5, 1987 Jun 01.
Article
in En
| MEDLINE
| ID: mdl-3663125
ABSTRACT
The codon usage of Tetrahymena thermophila and other ciliates deviates from the 'universal genetic code' in that UAA and probably UAG are not translational termination signals but code for glutamine. Therefore, translation in vitro of mRNA from Tetrahymena in a reticulocyte lysate is prematurely terminated if a UAA or UAG triplet is present in the reading frame of the mRNA. We show that the addition of a subcellular fraction from Tetrahymena thermophila enables a rabbit reticulocyte lysate to translate Tetrahymena mRNAs into full-sized proteins. The activity of the subcellular fraction is shown to depend on the combined function of a protein component(s) and a tRNA(s). The subcellular fraction is easily prepared and its usefulness for the identification of isolated mRNAs from Tetrahymena by their translation products in vitro is demonstrated.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Tetrahymena
/
Protein Biosynthesis
/
Codon
/
RNA, Messenger
Limits:
Animals
Language:
En
Journal:
Biochem J
Year:
1987
Document type:
Article
Affiliation country:
Denmark