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Single-cell RNA sequencing identifies a population of human liver-type ILC1s.
Krämer, Benjamin; Nalin, Ansel P; Ma, Feiyang; Eickhoff, Sarah; Lutz, Philipp; Leonardelli, Sonia; Goeser, Felix; Finnemann, Claudia; Hack, Gudrun; Raabe, Jan; ToVinh, Michael; Ahmad, Sarah; Hoffmeister, Christoph; Kaiser, Kim M; Manekeller, Steffen; Branchi, Vittorio; Bald, Tobias; Hölzel, Michael; Hüneburg, Robert; Nischalke, Hans Dieter; Semaan, Alexander; Langhans, Bettina; Kaczmarek, Dominik J; Benner, Brooke; Lordo, Matthew R; Kowalski, Jesse; Gerhardt, Adam; Timm, Jörg; Toma, Marieta; Mohr, Raphael; Türler, Andreas; Charpentier, Arthur; van Bremen, Tobias; Feldmann, Georg; Sattler, Arne; Kotsch, Katja; Abdallah, Ali T; Strassburg, Christian P; Spengler, Ulrich; Carson, William E; Mundy-Bosse, Bethany L; Pellegrini, Matteo; O'Sullivan, Timothy E; Freud, Aharon G; Nattermann, Jacob.
Affiliation
  • Krämer B; Department of Internal Medicine I, University of Bonn, 53127 Bonn, Germany; German Center for Infection Research (DZIF), 53127 Bonn, Germany. Electronic address: benjamin.kraemer@ukbonn.de.
  • Nalin AP; Medical Scientist Training Program, The Ohio State University, Columbus, OH 43210, USA; Biomedical Sciences Graduate Program, The Ohio State University, Columbus, OH 43210, USA.
  • Ma F; Molecular Cell and Developmental Biology, College of Life Sciences, University of California Los Angeles, Los Angeles, CA 90095, USA.
  • Eickhoff S; Institute of Experimental Oncology (IEO), Medical Faculty, University Hospital Bonn, University of Bonn, 53127 Bonn, Germany.
  • Lutz P; Department of Internal Medicine I, University of Bonn, 53127 Bonn, Germany; German Center for Infection Research (DZIF), 53127 Bonn, Germany.
  • Leonardelli S; Institute of Experimental Oncology (IEO), Medical Faculty, University Hospital Bonn, University of Bonn, 53127 Bonn, Germany.
  • Goeser F; Department of Internal Medicine I, University of Bonn, 53127 Bonn, Germany; German Center for Infection Research (DZIF), 53127 Bonn, Germany.
  • Finnemann C; Department of Internal Medicine I, University of Bonn, 53127 Bonn, Germany; German Center for Infection Research (DZIF), 53127 Bonn, Germany.
  • Hack G; Department of Internal Medicine I, University of Bonn, 53127 Bonn, Germany; German Center for Infection Research (DZIF), 53127 Bonn, Germany.
  • Raabe J; Department of Internal Medicine I, University of Bonn, 53127 Bonn, Germany; German Center for Infection Research (DZIF), 53127 Bonn, Germany.
  • ToVinh M; Department of Internal Medicine I, University of Bonn, 53127 Bonn, Germany; German Center for Infection Research (DZIF), 53127 Bonn, Germany.
  • Ahmad S; Department of Internal Medicine I, University of Bonn, 53127 Bonn, Germany; German Center for Infection Research (DZIF), 53127 Bonn, Germany.
  • Hoffmeister C; Department of Internal Medicine I, University of Bonn, 53127 Bonn, Germany; German Center for Infection Research (DZIF), 53127 Bonn, Germany.
  • Kaiser KM; Department of Internal Medicine I, University of Bonn, 53127 Bonn, Germany; German Center for Infection Research (DZIF), 53127 Bonn, Germany.
  • Manekeller S; Department of Surgery, University of Bonn, 53127 Bonn, Germany.
  • Branchi V; Department of Surgery, University of Bonn, 53127 Bonn, Germany.
  • Bald T; Institute of Experimental Oncology (IEO), Medical Faculty, University Hospital Bonn, University of Bonn, 53127 Bonn, Germany.
  • Hölzel M; Institute of Experimental Oncology (IEO), Medical Faculty, University Hospital Bonn, University of Bonn, 53127 Bonn, Germany.
  • Hüneburg R; Department of Internal Medicine I, University of Bonn, 53127 Bonn, Germany.
  • Nischalke HD; Department of Internal Medicine I, University of Bonn, 53127 Bonn, Germany.
  • Semaan A; Department of Surgery, University of Bonn, 53127 Bonn, Germany.
  • Langhans B; Department of Internal Medicine I, University of Bonn, 53127 Bonn, Germany; German Center for Infection Research (DZIF), 53127 Bonn, Germany.
  • Kaczmarek DJ; Department of Internal Medicine I, University of Bonn, 53127 Bonn, Germany.
  • Benner B; Biomedical Sciences Graduate Program, The Ohio State University, Columbus, OH 43210, USA.
  • Lordo MR; Medical Scientist Training Program, The Ohio State University, Columbus, OH 43210, USA; Biomedical Sciences Graduate Program, The Ohio State University, Columbus, OH 43210, USA.
  • Kowalski J; Nationwide Children's Hospital, Columbus, OH, USA.
  • Gerhardt A; College of Medicine, University of Cincinnati, Cincinnati, OH 45221, USA.
  • Timm J; Institute of Virology, University of Duesseldorf, 40225 Düsseldorf, Germany.
  • Toma M; Department of Pathology, University of Bonn, 53127 Bonn, Germany.
  • Mohr R; Department of Internal Medicine I, University of Bonn, 53127 Bonn, Germany.
  • Türler A; General and Visceral Surgery, Johanniter Hospital, 53113 Bonn, Germany.
  • Charpentier A; Department of Otorhinolaryngology/Head and Neck Surgery, University of Bonn, 53127 Bonn, Germany; Department of Otorhinolaryngology, Head and Neck Surgery, Medical Faculty, University of Cologne, 50931 Cologne, Germany.
  • van Bremen T; Department of Otorhinolaryngology/Head and Neck Surgery, University of Bonn, 53127 Bonn, Germany.
  • Feldmann G; Department of Internal Medicine III, University of Bonn, 53127 Bonn, Germany.
  • Sattler A; Clinic for Surgery, Transplant Immunology Lab, Charité University Hospital Berlin, 10117 Berlin, Germany.
  • Kotsch K; Clinic for Surgery, Transplant Immunology Lab, Charité University Hospital Berlin, 10117 Berlin, Germany.
  • Abdallah AT; Interdisciplinary Center for Clinical Research, RWTH Aachen University, 52074 Aachen, Germany.
  • Strassburg CP; Department of Internal Medicine I, University of Bonn, 53127 Bonn, Germany.
  • Spengler U; Department of Internal Medicine I, University of Bonn, 53127 Bonn, Germany; German Center for Infection Research (DZIF), 53127 Bonn, Germany.
  • Carson WE; Division of Surgical Oncology, Department of Surgery, Comprehensive Cancer Center and The James Cancer Hospital and Solove Research Institute, Columbus, OH 43210, USA.
  • Mundy-Bosse BL; Division of Hematology, Department of Internal Medicine, Comprehensive Cancer Center, The James Cancer Hospital and Solove Research Institute, The Ohio State University, Columbus, OH 43210, USA.
  • Pellegrini M; Molecular Cell and Developmental Biology, College of Life Sciences, University of California Los Angeles, Los Angeles, CA 90095, USA.
  • O'Sullivan TE; Department of Microbiology, Immunology, and Molecular Genetics, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, CA 900953, USA.
  • Freud AG; Department of Pathology, Comprehensive Cancer Center, The James Cancer Hospital and Solove Research Institute, The Ohio State University, Columbus, OH 43210, USA. Electronic address: aharon.freud@osumc.edu.
  • Nattermann J; Department of Internal Medicine I, University of Bonn, 53127 Bonn, Germany; German Center for Infection Research (DZIF), 53127 Bonn, Germany.
Cell Rep ; 42(1): 111937, 2023 01 31.
Article in En | MEDLINE | ID: mdl-36640314
ABSTRACT
Group 1 innate lymphoid cells (ILCs) comprise a heterogeneous family of cytotoxic natural killer (NK) cells and ILC1s. We identify a population of "liver-type" ILC1s with transcriptional, phenotypic, and functional features distinct from those of conventional and liver-resident NK cells as well as from other previously described human ILC1 subsets. LT-ILC1s are CD49a+CD94+CD200R1+, express the transcription factor T-BET, and do not express the activating receptor NKp80 or the transcription factor EOMES. Similar to NK cells, liver-type ILC1s produce IFN-γ, TNF-α, and GM-CSF; however, liver-type ILC1s also produce IL-2 and lack perforin and granzyme-B. Liver-type ILC1s are expanded in cirrhotic liver tissues, and they can be produced from blood-derived ILC precursors in vitro in the presence of TGF-ß1 and liver sinusoidal endothelial cells. Cells with similar signature and function can also be found in tonsil and intestinal tissues. Collectively, our study identifies and classifies a population of human cross-tissue ILC1s.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Lymphocytes / Immunity, Innate Type of study: Prognostic_studies Limits: Humans Language: En Journal: Cell Rep Year: 2023 Document type: Article
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Lymphocytes / Immunity, Innate Type of study: Prognostic_studies Limits: Humans Language: En Journal: Cell Rep Year: 2023 Document type: Article