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Enriched Peripheral Blood-Derived Mononuclear Cells for Treating Knee Osteoarthritis.
Chuang, Chang-Han; Kuo, Chi-Chung; Chiang, Yueh-Feng; Lee, Pei-Yuan; Wang, Fu-Hui; Hsieh, Chia-Ying; Shen, Ching-I; Chung, Yu-Hsuan; Lee, Kuan-Der; Wu, Shih-Fang; Su, Hong-Lin; Lin, Chih-Lung.
Affiliation
  • Chuang CH; Department of Orthopedics, Show Chwan Memorial Hospital, Changhua, Taiwan.
  • Kuo CC; PhD Program in Translational Medicine, National Chung Hsing University, Taichung, Taiwan.
  • Chiang YF; Rong Hsing Research Center for Translational Medicine, National Chung Hsing University, Taichung, Taiwan.
  • Lee PY; Department of Neurology, Taichung Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, Taichung, Taiwan.
  • Wang FH; School of Post-Baccalaureate Chinese Medicine, Tzu Chi University, Hualien, Taiwan.
  • Hsieh CY; Department of Orthopedics, Taichung Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, Taichung.
  • Shen CI; Department of Orthopedics, Show Chwan Memorial Hospital, Changhua, Taiwan.
  • Chung YH; DuoGenic StemCells Corporation, Taichung, Taiwan.
  • Lee KD; DuoGenic StemCells Corporation, Taichung, Taiwan.
  • Wu SF; DuoGenic StemCells Corporation, Taichung, Taiwan.
  • Su HL; Department of Orthopedics, Show Chwan Memorial Hospital, Changhua, Taiwan.
  • Lin CL; PhD Program in Translational Medicine, National Chung Hsing University, Taichung, Taiwan.
Cell Transplant ; 32: 9636897221149445, 2023.
Article in En | MEDLINE | ID: mdl-36661223
Osteoarthritis (OA) is a common chronic skeletal disease in the elderly. There is no effective therapy to reverse disease severity and knee OA (KOA) progression, particularly at the late stage. This study aims to examine the effect of peripheral blood-derived mononuclear cells (PBMNCs) on pain and motor function rescue in patients with Kellgren-Lawrence (KL) grade II to IV KOA. Participants received one intra-articular (IA) injection of autologous PBMNCs. The mononuclear cells were isolated from peripheral blood, enriched by a specialized medium (MoFi medium), and separated by Ficoll-Paque solution. The isolated and enriched PBMNCs could differentiate into M1 and M2 macrophages in vitro. The in vivo anti-inflammatory effect of the PBMNCs was similar to that of bone marrow mesenchymal stem cells, evaluated by complete Freund's adjuvant-induced arthritis in rodents. A single-arm and open-label pilot study showed that patients' knee pain and motor dysfunction were significantly attenuated after the cell transplantation, assessed by visual analogue scale (VAS) and Knee injury and Osteoarthritis Outcome Score (KOOS) at 6 and 12 months post-treatment. Notably, the therapeutic effect of the PBMNCs treatment can be stably maintained for 24 months, as revealed by the KOOS scores. These preclinical and pilot clinical data suggest that IA injection of MoFi-PBMNCs might serve as a novel medical technology to control the pain and the progress of KOA.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Osteoarthritis, Knee Limits: Humans Language: En Journal: Cell Transplant Journal subject: TRANSPLANTE Year: 2023 Document type: Article Affiliation country: Taiwan Country of publication: United States

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Osteoarthritis, Knee Limits: Humans Language: En Journal: Cell Transplant Journal subject: TRANSPLANTE Year: 2023 Document type: Article Affiliation country: Taiwan Country of publication: United States