Longitudinal monitoring of pancreatic islet damage in streptozotocin-treated mice with optical coherence microscopy.
Biomed Opt Express
; 14(1): 54-64, 2023 Jan 01.
Article
in En
| MEDLINE
| ID: mdl-36698658
ABSTRACT
Pancreatic islets regulate glucose homeostasis in the body, and their dysfunction is closely related to diabetes. Islet transplantation into the anterior chamber of the eye (ACE) was recently developed for both in vivo islet study and diabetes treatment. Optical coherence microscopy (OCM) was previously used to monitor ACE transplanted islets in non-obese diabetic (NOD) mice for detecting autoimmune attack. In this study, OCM was applied to streptozotocin (STZ)-induced diabetic mouse models for the early detection of islet damage. A custom extended-focus OCM (xfOCM) was used to image islet grafts in the ACE longitudinally during STZ-induced beta cell destruction together with conventional bright-field (BF) imaging and invasive glucose level measurement. xfOCM detected local structural changes and vascular degradation during the islet damage which was confirmed by confocal imaging of extracted islet grafts. xfOCM detection of islet damage was more sensitive than BF imaging and glucose measurement. Longitudinal xfOCM images of islet grafts were quantitatively analyzed. All these results showed that xfOCM could be used as a non-invasive and sensitive monitoring method for the early detection of deficient islet grafts in the ACE with potential applications to human subjects.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Type of study:
Screening_studies
Language:
En
Journal:
Biomed Opt Express
Year:
2023
Document type:
Article