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Enhanced detoxification via Cyt-P450 governs cross-tolerance to ALS-inhibiting herbicides in weed species of Centaurea.
Palma-Bautista, Candelario; Vázquez-García, José G; de Portugal, Joao; Bastida, Fernando; Alcántara-de la Cruz, Ricardo; Osuna-Ruiz, Maria D; Torra, Joel; De Prado, Rafael.
Affiliation
  • Palma-Bautista C; Agroforestry and Plant Biochemistry, Proteomics and Systems Biology, Department of Biochemistry and Molecular Biology, University of Cordoba, UCO-CeiA3, 14014, Cordoba, Spain.
  • Vázquez-García JG; Agroforestry and Plant Biochemistry, Proteomics and Systems Biology, Department of Biochemistry and Molecular Biology, University of Cordoba, UCO-CeiA3, 14014, Cordoba, Spain. Electronic address: z82vagaj@uco.es.
  • de Portugal J; Biosciences Department, Polytechnic Institute of Beja, Beja, Portugal; VALORIZA-Research Centre for Endogenous Resource Valorization, Polytechnic Institute of Portalegre, Portalegre, Portugal.
  • Bastida F; Department of Agroforestry Sciences, Campus El Carmen, University of Huelva, 21007, Huelva, Spain.
  • Alcántara-de la Cruz R; Centro de Ciências da Natureza, Campus Lagoa Do Sino, Universidade Federal de São Carlos, Buri, 18290-000, Brazil.
  • Osuna-Ruiz MD; Plant Protection Department, Extremadura Scientific and Technological Research Center (CICYTEX), Ctra. de AV, Km 372, Badajoz, 06187, Guadajira, Spain.
  • Torra J; Department D'Hortofructicultura, Botànica i Jardineria, Agrotecnio-CERCA Center, Universitat de Lleida, Lleida, Spain.
  • De Prado R; Agroforestry and Plant Biochemistry, Proteomics and Systems Biology, Department of Biochemistry and Molecular Biology, University of Cordoba, UCO-CeiA3, 14014, Cordoba, Spain.
Environ Pollut ; 322: 121140, 2023 Apr 01.
Article in En | MEDLINE | ID: mdl-36706859
Centaurea is a genus of winter weeds with a similar life cycle and competitive traits, which occurs in small-grains production fields in the central-southern of the Iberian Peninsula. However, most of herbicides recommended for weed management in wheat show poor control of Centaurea species. This study summarizes the biology, herbicide tolerance to acetolactate synthase (ALS) inhibitors, and recommended chemical alternatives for the control of Centaurea species. Four species (C. cyanus L., C. diluta Aiton, C. melitensis L. and C. pullata L. subsp. baetica Talavera), taxonomically characterized, were found as the main important broadleaf weeds in small-grains production fields of the Iberian Peninsula. These species showed innate tolerance to tribenuron-methyl (TM), showing LD50 values (mortality of 50% of a population) higher than the field dose of TM (20 g ai ha-1). The order of tolerance was C. diluta (LD50 = 702 g ha-1) ≫ C. pullata (LD50 = 180 g ha-1) ≫ C. cyanus (LD50 = 65 g ha-1) > C. melitensis (LD50 = 32 g ha-1). Centaurea cyanus and C. melitensis presented higher foliar retention (150-180 µL herbicide solution), absorption (14-28%) and subsequent translocation (7-12%) of TM with respect to the other two species. Centaurea spp. plants were able to metabolize 14C-TM into non-toxic forms (hydroxylated OH-metsulfuron-methyl and conjugated-metsulfuron-methyl), with cytochrome P450 (Cyt-P450) monooxygenases being responsible for herbicide detoxification. Centaurea cyanus and C. mellitensis metabolized up to 25% of TM, while C. diluta and C. pullata metabolized more than 50% of the herbicide. Centaurea species showed 80-100% survival when treated with of florasulam, imazamox and/or metsulfuron-methyl, i.e., these weeds present cross-tolerance to ALS inhibitors. In contrast, auxin mimics herbicides (2,4-D, clopyralid, dicamba, fluroxypir and MCPA) efficiently controlled the four Centaurea species. In addition, the mixture of ALS-inhibitors and auxin mimics also proved to be an interesting alternative for the control of Centaurea. These results show that plants of the genus Centaurea found in the winter cereal fields of the Iberian Peninsula have an innate tolerance to TM and cross-resistance to other ALS-inhibiting herbicides, governed by reduced absorption and translocation, but mainly by the metabolization of the herbicide via Cyt-P450.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Acetolactate Synthase / Centaurea / Herbicides Language: En Journal: Environ Pollut Journal subject: SAUDE AMBIENTAL Year: 2023 Document type: Article Affiliation country: Spain Country of publication: United kingdom

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Acetolactate Synthase / Centaurea / Herbicides Language: En Journal: Environ Pollut Journal subject: SAUDE AMBIENTAL Year: 2023 Document type: Article Affiliation country: Spain Country of publication: United kingdom