Pueraria protein extract inhibits melanogenesis and promotes melanoma cell apoptosis through the regulation of MITF and mitochondrialrelated pathways.
Mol Med Rep
; 27(3)2023 Mar.
Article
in En
| MEDLINE
| ID: mdl-36734267
ABSTRACT
Pueraria Lobata Radix (P. Lobata Radix) is an edible traditional Chinese medicine that contains various active compounds. Proteins from P. Lobata Radix have become the subject of increased interest in recent years. In evaluating the whitening effect on the skin, the present study found that the P. Lobata Radix watersoluble total protein extract (PLP) had the strongest inhibitory effect on tyrosinase activity. In the present study, the antimelanogenic effect of PLP and the inhibitory effect on B16 melanoma cells were investigated. PLP significantly reduced the tyrosinase activity and melanin content in B16 melanoma cells. Mechanistically, PLP inhibited melanogenesis by decreasing the expression of tyrosinase, tyrosinaserelated protein (TRP)1 and TRP2 through downregulation of the microphthalmiaassociated transcription factor (MITF) gene, which was mediated by inhibition of p38 mitogenactivated protein kinase signaling. In addition, PLP inhibited cell viability and triggered apoptosis of B16 cells in a dosedependent manner. Exposure to PLP reduced the mitochondrial membrane potential (MMP) and decreased ATP generation, leading to mitochondriarelated apoptosis of B16 melanoma cells. The expression levels of succinate dehydrogenase (SDH) and its two related subunits (SDHA and SDHB) were downregulated significantly by PLP, which may be associated with the regulation of mitochondrial energy metabolism by PLP. These results may explain why MMP collapse and reduced ATP generation were observed in B16 melanoma cells treated with PLP. Finally, the present study demonstrated that the inhibition of melanin synthesis by PLP was correlated with the regulation of antioxidant enzymes to reduce reactive oxygen species levels. These results suggested that PLP inhibits melanogenesis by downregulating the expression of MITFrelated melanogenic enzymes and triggering apoptosis through mitochondriarelated pathways.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Melanoma, Experimental
/
Pueraria
Limits:
Animals
Language:
En
Journal:
Mol Med Rep
Year:
2023
Document type:
Article